Identification of Three Novel Unique Proteins in Seed Oil Bodies of Sesame
スポンサーリンク
概要
- 論文の詳細を見る
Plant seeds store triacylglycerols in discrete organelles called oil bodies. An oil body preserves a matrix of triacylglycerols surrounded by a monolayer of phospholipids embedded with abundant structural proteins termed oleosins and probably some uninvestigated minor proteins of higher molecular mass. Three polypeptides of 27, 37, and 39 kDa(temporarily denominated as Sop1, Sop2, and Sop3) were regularly co-purified with seed oil bodies of sesame. Comparison of amino acid composition indicated that they were substantially less hydrophobic than the known oleosins, and thus should not be aggregated multimers of oleosins. The results of immuno-recognition to sesame proteins extracted from subcellular fractions of mature seeds, various tissues, and oil bodies purified from different stages of seed formation revealed that these three polypeptides were unique proteins gathered in oil bodies, accompanying oleosins and triacylglycerols, during the active assembly of the organelles in maturing seeds. Both in vivo and in intro, immunofluorescence labeling using secondary antibodies conjugated with FITC(fluorescein isothiocyanate) confirmed the localization of these three polypeptides in oil bodies.
- 日本植物生理学会の論文
著者
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TZEN Jason
Graduate Institute of Agricultural Biotechnology National Chung-Hsing university
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Tzen J
National Chung Hsing Univ. Taichung Twn
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Tzen Jason
Graduate Inst. Of Biotechnology National Chung Hsing Univ.
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TAI Sorgan
Graduate Institute of Agricultural Biotechnology, National Chung-Hsing University
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PENG Chi-Chung
Graduate Institute of Agricultural Biotechnology, National Chung-Hsing University
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Peng C‐c
Graduate Institute Of Agricultural Biotechnology National Chung-hsing University
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Tai Sorgan
Graduate Institute Of Agricultural Biotechnology National Chung-hsing University
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Peng Chi-chung
Graduate Institute Of Agricultural Biotechnology National Chung-hsing University
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Chen Emily
Graduate Institute Of Agricultural Biotechnology National Chung-hsing University
関連論文
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- Cloning and Expression of a Seed-Specific Metallothionein-Like Protein from Sesame
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- Enhanced Methionine and Cysteine Levels in Transgenic Rice Seeds by the Accumulation of Sesame 2S Albumin(Food & Nutrition Science)
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