Classification of the Single Oleosin Isoform and Characterization of Seed Oil Bodies in Gymnosperms

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概要

• 論文の詳細を見る

• Oleosins are hydrophobic proteins abundantly present in the oil bodies of plant seeds. Two immunologically distinct oleosins are present in seed oil bodies of diverse angiosperms, and classified as high and low M_r isoforms according to their relative molecular masses in each species. Only one putative oleosin was found in seed oil bodies of three representative gymnosperm species, Pinus koraiensis, Ginkgo biloba, and Cycas revoluta. The three gymnosperm oleosins were restricted to oil bodies, as detected on immuno-assaying. Immunological cross-recognition using antibodies against the three putative gymnosperm oleosins and those against the two (high and low M_r) rice oleosin isoforms suggests that the single oleosin of pine or ginkgo is immunologically related to the low M_r isoform of angiosperms, while the single cycad oleosin is immunologically distinct from both low and high M_r isoforms of angiosperms. Oil bodies were found in embryos and megagametophytes of these three species, as observed on electron microscopy. Seed oil bodies purified from these three gymnosperms maintained their integrity via electronegative repulsion and steric hindrance on the surface of the organelles. The compositions of the three essential constituents (neutral lipid, phospholipid and protein) in seed oil bodies from these three species were determined and compared with those calculated from the oil body model proposed in angiosperms. The results suggested that seed oil bodies of gymnosperms and angiosperms possess similar surface properties and structural organization.

• 日本植物生理学会の論文

著者

• WU Lawrence

  Graduate Institute of Agricultural Biotechnology National Chung-Hsing university

• TZEN Jason

  Graduate Institute of Agricultural Biotechnology National Chung-Hsing university

• Tzen Jason

  Graduate Inst. Of Biotechnology National Chung Hsing Univ.

• Wu L

  Graduate Institute Of Agricultural Biotechnology National Chung-hsing University

• Hou Roger

  Department of Entomology, National Chung Hsing University

• Hong Grace

  Graduate Institute of Agricultural Biotechnology, National Chung-Hsing University

• Hong Grace

  Graduate Institute Of Agricultural Biotechnology National Chung-hsing University

• Hou Roger

  Department Of Entomology National Chung-hsing University

• Hou Roger

  Department Of Entomology National Chung Hsing University

関連論文

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• Classification of the Single Oleosin Isoform and Characterization of Seed Oil Bodies in Gymnosperms
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