Detection of Monodehydroascorbic Acid Radical in Sulfite-Treated Leaves and Mechanism of its Formation
スポンサーリンク
概要
- 論文の詳細を見る
The aim of the present study is to detect the monodehy-droascorbic acid(MDA) radical in broad bean(Vicia faba L.) leaves which were treated by vacuum-infiltration in Na_2SO_3 solution and subsequent centrifugation(sulfite-treated leaves). When sulfite-treated leaves were illuminated with white light, the electron spin resonance(ESR) signal of MDA radical was observed. The level of the MDA radical depended on the concentration of sulfite that was used for vacuum-infiltration and on the light intensity of illumination. The formation of the MDA radical in sulfite-treated leaves was inhibited by DCMU or by replacement of air with N_2. Glycolaldehyde also inhibited the formation of MDA radical in sulfite-treated leaves. Catalase activity was decreased by the sulfite treatment without affecting significantly the activities of ascorbate peroxidase(AA-POX) and of peroxidase which preferentially oxidizes phenolics(PhOH-POX). From these results, we conclude that the formation of the MDA radical in sulfite-treated leaves is catalyzed by peroxidases using the H_2O_2 which is generated by photorespiration and the Mehler reaction.
- 日本植物生理学会の論文
著者
-
Veljovic-jovanovic Sonja
Kyushu Dental College
-
ONIKI Takayuki
Kyushu Dental College
-
TAKAHAMA Umeo
Kyushu Dental University
-
Takahama Umeo
Kyushu Dental College
関連論文
- Effects of the Encapsulation of Alkali Ions into Iron(III) Complexes with a Macrocyclic Ring on Magnetic Properties
- Change in oxidation state of manganese atoms in kraft pulp during biological bleaching with white-rot fungus Phanerochaete sordida YK-624
- Oxidation of Quercetin by Salivary Components II. Effects of Quercetin on Reactive Oxygen Metabolism by Salivary Polymorphonuclear Leukocytes
- Oxidation of Quercetin by Salivary Components I. Salivary Peroxidase-Dependent Oxidation of Quercetin and Characterization of the Oxidation Products
- Quercetin Glucosides are Hydrolyzed to Quercetin in Human Oral Cavity to Participate in Peroxidase-Dependent Scavenging of Hydrogen Peroxide
- Flavonoids and Some Other Phenolics as Substrates of Peroxidase: Physiological Significance of the Redox Reactions
- Effects of Ascorbate on the Oxidation of Derivatives of Hydroxycinnamic Acid and the Mechanism of Oxidation of Sinapic Acid by Cell Wall-Bound Peroxidases : GENES STRUCTURE AND EXPRESSION
- A Possible Mechanism for the Oxidation of Sinapyl Alcohol by Peroxidase-Dependent Reactions in the Apoplast: Enhancement of the Oxidation by Hydroxycinnamic Acids and Components of the Apoplast
- Detection of Monodehydroascorbic Acid Radical in Sulfite-Treated Leaves and Mechanism of its Formation
- AGE-DEPENDENT CHANGES IN LEVELS OF ASCORBIC ACID AND CHLOROGENIC ACID, AND ACTIVITIES OF PEROXIDASE AND SUPEROXIDE DISMUTASE IN THE APOPLAST OF TOBACCO LEAVES
- Age-Dependent Changes in Levels of Ascorbic Acid and Chlorogenic Acid, and Activities of Peroxidase and Superoxide Dismutase in the Apoplast of Tobacco leaves : Mechanism of the Oxidation of Chlorogenic Acid in the Apoplast
- 3, 4-Dihydroxyphenylalanine Is Oxidized by Phenoxyl Radicals of Hydroxycinnamic Acid Esters in Leaves of Vicia faba L.
- PRODUCTION OF PHENOLICS AND ACTIVE OXYGEN SPECIES AS ANTIMICROBIAL COMPOUNDS DURING MATURING OF ONIONSCALES
- THE MECHANISM OF PEROXIDASE-DEPENDENT OXIDATION OF 3, 4-DIHYDROXYPHENYLALANINE IN MESOPHYLL CELLS OF Vicia Faba
- Phenolic Components of Brown Scales of Onion Bulbs Produce Hydrogen Peroxide by Autooxidation
- Free radicals produced by the oxidation of gallic acid and catechin derivatives
- Enhancement of Peroxidase-Dependent Oxidation of Sinapyl Alcohol by an Apoplastic Component, 4-Coumaric Acid Ester Isolated from Epicotyls of Vigna angularis L.
- Distribution of Flavonols and Enzymes Participating in the Metabolism in Onion Bulbs : Mechanism of Accumulation of Quercetin and Its Glucosides in the Abaxial Epidermis
- Quercetin and the Glucosides Inhibit Nitration of a Salivary Component 4-Hydroxyphenylacetic Acid Catalyzed by Salivary Polymorphonuclear Leukocytes
- QUERCETIN IS A NATURAL ELECTRON DONOR TO PEROXIDASE IN ONION BULBS
- Oxidation of Quercetin by Carotenoid Radical Generated in Illuminated Spinach Chloroplasts : The Effect of Ascorbate on Quercetin Oxidation
- Generation of Hydroxyl Radical in Isolated Pea Root Cell Wall, and the Role of Cell Wall-Bound Peroxidase, Mn-SOD and Phenolics in Their Production
- Physical Characteristic and Molecular Structure of Spin-Crossover Iron(III) Complexes of Monoclinic Form with Hexadentate Ligands Derived from Triethylenetetramine and Salicylaldehyde (Fe(sal2trien))BPh4acetone.