Involvement of a Calcium-Dependent Protein Kinase in Hypoosmotic Turgor Regulation in a Brackish Water Characeae Lamprothamnium succinctum
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概要
- 論文の詳細を見る
Calcium ion is a key messenger in turgor regulation of internodal cells of Lamprothamnium succinctum in response to hypoosmotic treatment. An increase in the concentration of cytosolic free calcium ion ([Ca^<2+>]_c) is prerequisite for the turgor regulation [Okazaki and Tazawa (1990) J. Membr. Biol. 114: 189]. We examined whether or not a calcium-dependent protein kinase (CDPK) is involved in the Ca^<2+>-mediated turgor regulation of Lamprothamnium cells. A 53-kDa CDPK which phosphorylated preferentially histone H1 but poorly myelin basic protein or casein, was detected in the cell extract of Lamprothamnium by an in-gel protein kinase assay. This protein kinase was detected by Western blotting and was immunoprecipitated using an anti-Dunaliella tertiolecta CDPK antibody which can neutralize the Dunaliella CDPK activity [Yuasa et al. (1995) Plant Cell Physiol. 36: 699]. The 53-kDa CDPK was partially purified from Lamprothamnium and its activity was shown to be inhibited by the antibody and K-252a, a protein kinase inhibitor. Microinjection of the antibody into the cytosol of Lamprothamnium cells inhibited the decrease in turgor pressure in response to hypoosmotic treatment. However, a transient increase in [Ca^<2+>]_c, which was suggested by a transient reduction of the velocity of cytoplasmic streaming, was induced in anti-body-injected cells after hypoosmotic treatment. Turgor regulation upon hypoosmotic treatment was inhibited when the cells were treated with K-252a. These results imply that CDPK of Lamprothamnium functions at a down-stream position of Ca^<2+>-mobilization in processing turgor regulation in response to hypoosmotic treatment.
- 日本植物生理学会の論文
著者
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Iwasaki Naohiko
Dept. Biol. Osaka Med. College
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Iwasaki Naohiko
Department Of Biology Osaka Medical College
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Iwasaki Naohiko
Osaka Medical College Department Of Biology
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Iwasaki Naohiko
Dept. Biol. Osaka Med. Coll.
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MUTO Shoshi
Nagoya University Bioscience Center, Nagoya University
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Muto S
Nagoya University Bioscience Center Nagoya University:graduate School Of Agricultural Sciences Nagoy
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Yuasa T
Nagoya University Bioscience Center Nagoya University
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Yuasa Takashi
Nagoya University Bioscience Center
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Okazaki Yoshiji
Department of Biology, Faculty of Science, University of Tokyo
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Okazaki Yoshiji
Department Of Biology Faculty Of Science University Of Tokyo
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Muto Shoshi
Nagoya University Bioscience Center
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Yuasa Takashi
Graduate School Of Bioagricultural Sciences Nagoya University
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Muto Shoshi
Nagoya Univ. Bioscience Center And Graduate School Of Bioagricultural Sciences
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Okazaki Yoshiji
Dept. Biol., Osaka Med. College
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