Irradiance-Dependent Changes in the Size and Composition of the Chlorophyll a-b Light-Harvesting Complex in the Green Alga Dunaliella salina
スポンサーリンク
概要
- 論文の詳細を見る
Irradiance-dependent adjustments in the size and composition of the Chl a-b light-harvesting protein complex (LHC-II) were investigated in the green alga Dunaliella salina. Cells grown under low irradiance (100 μmol photons m^<-2> s^<-1>) had a low Chl a/Chl b ratio, and an abundance of cellular Chl and LHC-II apoproteins compared to cells grown under high light intensity (2,200 μmol photons m^<-2> s^<-1>). In low-light grown cells, four apoproteins of the LHC-II migrating to 32, 31, 30 and 28.5 kDa were termed according to their apparent molecular mass as LHC-II-1, LHC-II-2, LHC-II-3 and LHC-II-4, respectively. In thylakoid membranes from high-light grown cells, LHC-II-1 was practically missing and LHC-II-2 was greatly depleted. On a cell basis, the steady-state amount of total LHC-II apoprotein in high-light grown cells, measured either from the intensity of protein staining by Coomassie brilliant blue or from Western blotting, was 5-7% of that in low-light. In vivo rates of apoprotein biosynthesis were measured from the time course of [^<35>S]sulfate incorporation into LHC-II. On a cell basis, the rate of biosynthesis in high-light grown cells was about 20% of that in low-light grown cells. Thus, in high-light grown cells, the rate of LHC-II apoprotein biosynthesis was 〜3 times greater than the steady-state amounts of LHC-II present in thylakoids. The results suggest discrete irradiance-dependent transcriptional and posttranslational regulation steps that define the amount and composition of the LHC-II in chloroplasts.
- 日本植物生理学会の論文
著者
-
Melis Anastasios
Department Of Plant & Microbial Biology University Of California
-
Tanaka Ayumi
Department Of Food And Nutrition Japan Women's University
-
Tanaka Ayumi
Department Of Botany Faculty Of Science Kyoto University
-
Tanaka Ayumi
Department Of Plant Biology University Of California:(permanent)department Of Botany Faculty Of Scie
関連論文
- Indentation Size Effect for the Hardness of Refractory Carbides
- Thylakoid Membrane Development and Differentiation : Assembly of the Chlorophyll a-b Light-Harvesting Complex and Evidence for the Origin of M_r=19,17.5 and 13.4kDa Proteins : MEMBRANES AND BIOENERGETICS
- Analysis of non-photochemical energy dissipating processes in wild type Dunaliella salina (green algae) and in zea1, a mutant constitutively accumulating zeaxanthin
- Up-Regulation of Photoprotection and PSII-Repair Gene Expression by Irradiance in the Unicellular Green Alga Dunaliella salina
- Chromatic Regulation in Chlamydomonas reinhardtii: Time Course of Photosystem Stoichiometry Adjustment Following a Shift in Growth Light Quality
- A Cytokinin-Repressed Gene in Cucumber for a bHLH Protein Homologue is Regulated by Light
- DNA INSERTIONAL MUTATION FOR THE ANALYSIS OF PHYSIOLOGICAL EVENTS IN CHLAMYDOMONAS REINHARDTII
- Effects of 5-Aminolevulinic Acid on the Accumulation of Chlorophyll b and Apoproteins of the Light-Harvesting Chlorophyll a/b-Protein Complex of Photosystem II
- Organization and Stability of Polypeptides Associated with the Chlorophyll a-b Light-Harvesting Complex of Photosystem-II : MEMBRANES AND BIOENERGETICS
- Absence of Lutein, Violaxanthin and Neoxanthin Affects the Functional Chlorophyll Antenna Size of Photosystem-II but not that of Photosystem-I in the Green Alga Chlamydomonas reinhardtii
- Photosystem II Damage and Repair Cycle in the Green Alga Dunaliella salina: Involvement of a Chloroplast-Localized HSP70
- Production of Novel Antioxidative Prenyl Naphthalen-ols by Combinational Bioconversion with Dioxygenase PhnA1A2A3A4 and Prenyltransferase NphB or SCO7190
- Changes of Photosystem Stoichiometry during Cell Growth in Dunaliella salina Cultures
- Localization and Characterization of a Novel 20 kDa Polypeptide in the Chloroplast of the Green Alga Dunaliella salina : MEMBRANES AND BIOENERGETICS: STRUCTURE AND FUNCTION OF CELLS
- Irradiance-Dependent Changes in the Size and Composition of the Chlorophyll a-b Light-Harvesting Complex in the Green Alga Dunaliella salina
- Identification and Characterization of a Novel Mutation M579Fs+75X in HERG