Thylakoid Membrane Development and Differentiation : Assembly of the Chlorophyll a-b Light-Harvesting Complex and Evidence for the Origin of M_r=19,17.5 and 13.4kDa Proteins : MEMBRANES AND BIOENERGETICS
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概要
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Pea plants were grown under intermittent illumination (ImL) conditions. The low dosage of light given to ImL plastids limited the rate of chlorophyll (Chl) a and Chl b biosynthesis and, therefore, it retarded the rate of photosynthetic unit formation and thylakoid membrane development. Depending on the developmental stage of the photosynthetic unit, ImL plastids had variable Chl a/Chl b ratios (2.7<Chl a/Chl b<20) and showed distinct intermediates in the assembly of the chlorophyll a-b light-harvesting complex (LHC) of photosystem-II (PSII). The results are consistent with a step-wise increment in the PSII antenna size involving three distinct forms of the PSII unit: (i) a PSII-core form with about 37 Chl a molecules; (ii) a PSII_β form containing the PSII-core and the LHC-II-inner antenna with a total of about 130 Chl (a+b) molecules, and (iii) the mature PSII_α form containing PSII_β and the LHC-II-peripheral antenna with a total of 210-300 Chl (a+b) molecules. The thylakoid membrane contained polyptptide subunits b, c and d (the Lhcb1, 2 and 3 gene products, respectively) when only the LHC-II-inner was present. Polypeptide subunit a, (the apoprotein of the chlorophyll-protein known as CP29), along with increased amounts of b and c appeared later in the development of thylakoids, concomitant with the assembly of the LHC-II-peripheral. The results suggest that polypeptide sub-unit d has priority of assembly over subunit a. It is implied that, of all LHC-II constituent proteins, subunit d is most proximal to the PSII-core complex and that it serves as a linker in the transfer of excitation energy from the bulk LHC-II (subunits b and c) to the PSII-core. The work also addresses the origin of low-molecular-weight proteins (M_r = 19, 17.5 and 13.4 kDa) which co-isolate with intact developing plastids and whose abundance decreases during plastid development. Amino acid compositional and immunoblot analyses show a nuclear histone origin for these low-molecular-weight proteins and suggest co-isolation of histone-containing nuclear vesicles along with intact developing plastids.
- 日本植物生理学会の論文
著者
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Morrissey Peter
Department Of Plant Biology 411 Koshland Hall University Of California
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MAWSON Bruce
Department of Plant Biology, 411 Koshland Hall, University of California
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GOMEZ Amparo
Department of Plant Biology, 411 Koshland Hall, University of California
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MELIS Anastasios
Department of Plant Biology, 411 Koshland Hall, University of California
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Melis Anastasios
Department Of Plant Biology 411 Koshland Hall University Of California
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Melis Anastasios
Department Of Plant & Microbial Biology University Of California
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Gomez Amparo
Department Of Plant Biology 411 Koshland Hall University Of California
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Mawson B
Univ. California Ca Usa
関連論文
- Thylakoid Membrane Development and Differentiation : Assembly of the Chlorophyll a-b Light-Harvesting Complex and Evidence for the Origin of M_r=19,17.5 and 13.4kDa Proteins : MEMBRANES AND BIOENERGETICS
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