A Recombinant Rice 16.9-kDa Heat Shock Protein Can Provide Thermoprotection in Vitro : ENVIRONMENTAL AND STRESS RESPONSES : GENE STRUCTURE AND EXPRESSION

スポンサーリンク

概要

• 論文の詳細を見る

• It is difficult to obtain large amounts of purified low-molecular-mass heat shock proteins (LMM HSPs), which are unique to plants, for biochemical and physiological studies. Therefore, an attempt was made to produce such a HSP by applying recombinant DNA technology. We fused the cDNA for a rice class I 16.9-kDa HSP, pTSl, to the gene for glutathione S-transferase (GST) of Schistosoma japonicum and we obtained large amounts of the fusion protein from transformed Escherichia coli cells. In addition, we found that the 16.9-kDa HSP obtained by cleavage of the recombinant protein could also form a protein complex of 〜310 kDa under non-denaturing conditions as can the small, native, class I HSPs from heat-shocked rice seedlings. An assay in vitro to examine the thermoprotection of rice soluble proteins from heat denaturation revealed the strong stabilizing effect of the recombinant HSP.

• 日本植物生理学会の論文

著者

• Chang Pi-fang

  Department Of Botany National Taiwan University

• Yeh Ching-Hui

  Department of Botany, National Taiwan University

• Yeh Kai-Wun

  Department of Botany, National Taiwan University

• Wu Shu-Hsing

  Department of Botany, National Taiwan University

• Chen Yih-Ming

  Department of Botany, National Taiwan University

• Lin Chu-Yung

  Department of Botany, National Taiwan University

• Chen Y‐m

  Department Of Botany National Taiwan University

• Wo Shu-hsing

  Department Of Botany National Taiwan University

• Yeh Ching-hui

  Department Of Botany National Taiwan University

• Yeh Kai-wun

  Department Of Botany National Taiwan University

• Lin Chu-yung

  Department Of Botany National Taiwan University

関連論文

• A Recombinant Rice 16.9-kDa Heat Shock Protein Can Provide Thermoprotection in Vitro : ENVIRONMENTAL AND STRESS RESPONSES : GENE STRUCTURE AND EXPRESSION
• Immunological Kinship of Class I Low Molecular Weight Heat Shock Proteins and Themostabilization of Soluble Proteins in Vitro among Plants
• Solute Leakage in Soybean Seedlings under Various Heat Shock Regimes
• A DEAD-Box Protein, AtRH36, is Essential for Female Gametophyte Development and is Involved in rRNA Biogenesis in Arabidopsis
• Relationships among the three light-induced absorbance changes related to the reaction-center of photosystem II

スポンサーリンク