Solute Leakage in Soybean Seedlings under Various Heat Shock Regimes

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The leakage of solute from intact seedlings during incubation under various heat shock (HS) regimes was studied. Continuous HS at 40℃ did not induce leakage of amino acids, soluble sugars and electrolytes into the incubation medium, when compared with control incubation at 28℃. Continuous HS at 45℃ (lethal treatment) caused leak-age to increase continuously and linearly during a 5-h treatment period. However, brief HS at 47.5℃, (lethal treatment), unlike continuous HS at 45℃, induced leakage at a slower rate which reached a plateau within 2 to 3 h at 28℃. Preincubation for 2 h at 40℃ completely prevented the leakage caused by the brief HS at 47.5℃, but not that caused by continuous 45℃ HS. The amount of leakage during 2 h of incubation at 45℃ was reduced to half by 30 min preincubation at 40℃ and was reduced to a minimal level by l-h preincubation. Greater reduction of leakage at 45℃ HS was observed when an additional 4 h of incubation at 28℃ immediately followed the 40℃ preincubation. These results and previous findings (Lin et al. 1984) indicate that the synthesis and accumulation of HS proteins (HSPs) are important for preventing HS-induced leakage from the cells. One of the HSPs, 15 kD in size appeared to be associated with the plasma membrane.
日本植物生理学会の論文