Immunological Kinship of Class I Low Molecular Weight Heat Shock Proteins and Themostabilization of Soluble Proteins in Vitro among Plants

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概要

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• The antibody prepared against one of the soybean (Glycine max) 15 to 18 kDa heat shock proteins (HSPs) that cross-reacted with the 12 polypeptides of 15 to 18 kDa class I low molecular weight (LMW) HSPs in soybean, was also found to cross-react in Western blot analyses with the class I LMW HSPs of nine other plant species, i.e., mung bean, pea, cucumber, tobacco, Arabidopsis, rice, maize, wheat, and barley. An antibody raised from the 16.9 kDa rice HSP also cross-reacted with the same class I LMW HSPs of the ten plant species tested. HSPs-enriched fractions (70 to 100% ammonium sulfate saturation) prepared from mung bean and rice heat-shocked seedlings were able to thermostabilize the homologous soluble proteins, as we have shown previously in soybean. Up to 50% of the soluble proteins that are normally denatured by heating at 55℃ for 30 min was protected when an HSPs-enriched fraction was added to either mung bean or rice protein. Additionally, the HSPs-enriched fractions were exchangeable among these three plant species for thermostabilization. The protection provided by these HSPs-enriched fractions is effective mainly for membrane-associated proteins. In soybean depletion of the 15 to 18 kDa HSPs in the HSPs-enriched fraction resulted in the loss of the thermostabilizing ability and when the 15 to 18 kDa HSPs were recovered in this fraction, the thermostabilizing ability was again restored. Thus, the 15 to 18 kDa HSPs in plant, which shuttle between the cyioplasm and cellular organelles during heat shock (HS) and recovery from HS, are responsible for providing the thermostabilization.

• 日本植物生理学会の論文

著者

• Yeh Yung-chun

  Botany Department National Taiwan University

• Yeh Ching-Hui

  Department of Botany, National Taiwan University

• Lin Chu-Yung

  Department of Botany, National Taiwan University

• Chen Y‐m

  Department Of Botany National Taiwan University

• JINN Tsung

  Botany Department, National Taiwan University

• WO Shu-Hsing

  Botany Department, National Taiwan University

• YEH Ching-Hui

  Botany Department, National Taiwan University

• HSIEH Ming-Hsiun

  Botany Department, National Taiwan University

• CHEN Yih-Ming

  Botany Department, National Taiwan University

• LIN Chu-Yung

  Botany Department, National Taiwan University

• Wo Shu-hsing

  Department Of Botany National Taiwan University

• Yeh Ching-hui

  Department Of Botany National Taiwan University

• Jinn T‐l

  Botany Department National Taiwan University

• Lin Chu-yung

  Department Of Botany National Taiwan University

• Hsieh Ming-hsiun

  Botany Department National Taiwan University

関連論文

• A Recombinant Rice 16.9-kDa Heat Shock Protein Can Provide Thermoprotection in Vitro : ENVIRONMENTAL AND STRESS RESPONSES : GENE STRUCTURE AND EXPRESSION
• Immunological Kinship of Class I Low Molecular Weight Heat Shock Proteins and Themostabilization of Soluble Proteins in Vitro among Plants
• Solute Leakage in Soybean Seedlings under Various Heat Shock Regimes
• A DEAD-Box Protein, AtRH36, is Essential for Female Gametophyte Development and is Involved in rRNA Biogenesis in Arabidopsis

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