Studies on Elicitor-Signal Transduction Leading to Differential Expression of Defense Genes in Cultured Tobacco Cells : ENVIRONMENTAL AND STRESS RESPONSES : GENE STRUCTURE AND EXPRESSION
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概要
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To elucidate the mechanism of elicitor-inducible gene expression, we investigated the induction of the accumulation of mRNAs transcribed from defense genes that encode class I basic chitinase (BCHN) and class II acidic chitinase (ACHN), class I basic β-1,3-glucanase (GLN) and 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase (DAHPS) in tobacco cells treated with a fungal elicitor. The elicitor treatment was shown to increase the steady-state levels of mRNAs for BCHN and GLN, and for ACHN and DAHPS. Expression of these pairs of genes was induced by elicitor with different kinetics of mRNA accumulation and differentially sensitive to pharmacological agents. Increases in the extracellular concentration of Ca^<2^+> ions and the treatment with Ca^<2^+> ionophore induced the accumulation of ACHN and DAHPS mRNAs. Calcium channel blockers prevented elicitor-inducible increases in levels of BCHN and GLN mRNAs. Cycloheximide inhibited the elicitor-inducible accumulation of BCHN and GLN mRNAs. The elicitor-inducible accumulation of transcripts from these defense genes was inhibited in the presence of staurosporine. Expression of DAHPS was induced by jasmonate-related compounds but that of BCHN, ACHN and GLN was not. Constructs of fusions of the 5'-upstream sequences of genes for BCHN and GLN with the reporter gene for β-glucuronidase were found to be elicitor-responsive in transgenic cells. Thus, it appears that expression of the genes for BCHN and GLN is regulated primarily at the transcriptional level.
- 日本植物生理学会の論文
著者
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Fukuda Yuji
Plant Molecular Biology Laboratory Molecular Biology Department National Institute Of Bioscience And
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SHINSHI Hideaki
Plant Molecular Lab., National Institute of Bioscience and Human Technology
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SUZUKI Kaoru
Plant Mol. Biol. Lab., NIBH, AIST
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Shinshi H
Aist Miti Tsukuba Jpn
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Shinshi Hideaki
Plant Moecular Biology Laboratory Molecular Biology Department National Institute Of Bioscience And
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Suzuki Kaoru
Plant Molecular Biology Laboratory Molecular Biology Department National Institute Of Bioscience And
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Suzuki Kaoru
Plant Moecular Biology Laboratory Molecular Biology Department National Institute Of Bioscience And
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