Coordinated Activation of Chitinase Genes and Extracellular Alkalinization in Suspension-cultured Tobacco Cells

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概要

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• Rapid alkalinization of the culture medium in response to an elicitor was observed before the accumulation of transcripts of the chitinase genes. A correlation between the increase in extracellular pH and the induction of the chitinase genes was investigated. N,N'-Dicyclohexylcarbodiimide, an inhibitor of proton channels such as H^+-ATPase, and carbonyl cyanide m-chlorophenylhydrazone, a protonophore, also induced the expression of basic class I and acidic class II chitinase genes and the rapid alkalinization of the culture medium. Both the alkalinization of the culture medium and the induction of the basic class I chitinase genes in response to the elicitor were inhibited by fusicoccin. These inhibitory effects were also observed by the treatment with staurosporine, and by the treatments with La^<3+> ions or Gd^<3+> ions. By contrast, the elicitor-inducible accumulation of transcripts of the acidic class II chitinase genes was not prevented in the presence of these inhibitors.

• 社団法人日本農芸化学会の論文
• 1996-12-23

著者

• Fukuda Yuji

  Plant Molecular Biology Laboratory Molecular Biology Department National Institute Of Bioscience And

• Fukuda Y

  Plant Molecular Biology Laboratory Molecular Biology Department National Institute Of Bioscience And

関連論文

• Studies on Elicitor-Signal Transduction Leading to Differential Expression of Defense Genes in Cultured Tobacco Cells : ENVIRONMENTAL AND STRESS RESPONSES : GENE STRUCTURE AND EXPRESSION
• Coordinated Activation of Chitinase Genes and Extracellular Alkalinization in Suspension-cultured Tobacco Cells

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