Hydroxyl Radicals and a Thylakoid-Bound Endopeptidase are Involved in Light-and Oxygen-Induced Proteolysis in Oat Chloroplasts : ENVIRONMENTAL AND STRESS RESPONSES : PROTEINS, ENZYMES AND METABOLISM

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概要

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• The hypothesis that light- and oxygen-induced proteolysis in chloroplasts is mediated by active oxygen species was examined. In order to determine whether or not H_2O_2 and/or ^・OH radicals are involved in these degradative processes we compared the degradation of proteins in isolated oat chloroplasts exposed to white light at 80 W m^<-2> with that in chloroplasts incubated in darkness in the absence or presence of H_2O_2 Or a ^・OH-generating system composed by ascorbic acid, FeCl_3 and H_2O_2 (Asc-Fe-H_2O_2). Light enhanced the rate of degradation of at least 18 polypeptides, while proteolysis was, almost negligible in darkness in the abscence of additives. H_2O_2 had a very small effect. However, Asc-Fe-H_2O_2-treated chloroplasts in darkness showed a pattern of protein degradation almost identical to that observed in the light. A thylakoid-bound endopeptidase (EP), the activity of which increased under photooxidative environmental conditions and treatment with an ^・OH-generating system, was partially purified and characterized as a serine-type protease. Treatments with inhibitors of serine-type protease prevented both light- and Asc-Fe-H_2O_2-induced proteolysis. EP was more active against both soluble and membranous proteins that had been pretreated with Asc-Fe-H_2O_2 than against untreated proteins. It is proposed that a high dose of light irradiation promotes proteolysis by increasing the formation of ^・OH, which may modify proteins such that they become more susceptible to EP-catalyzed hydrolysis.

• 日本植物生理学会の論文

著者

• Trippi Victorio

  Laboratorio de Fisiologia Vegetal, Facultad de Ciencias E., F. y N., Universidad Nacional de Cordoba

• Trippi V

  Inst. Fitopatologia Y Fisiolgia Vegetal Cordoba Arg

• Trippi Victorio

  Instituto De Fitopatologia Y Fisiologia Vegetal(iffive-inta)

• Trippi Victorio

  Laboratorio De Fisiologfa Vegetal

• Lascano H.ramiro

  Instituto De Fitopatologia Y Fisiologia Vegetal(iffive-inta)

• Casano L

  Departamento De Biologia Vegetal Universidad De Alcala De Henares

• Casano Leonardo

  Laboratorio De Fisiologia Vegetal (imbiv) Facultad De Ciencias Exactas Fisicas Y Naturales Universid

• Lascano H.Ramiro

  Laboratorio de Fisiologla Vegetal, Facultad de Ciencias Exactas, Fisicas y Naturales, Universidad Na

• Trippi V.

  Laboratorio De Fisiologia Vegetal Imviv. Facultad De C. E. F. Y Naturales Universidad Nacional De Co

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