Isolation and Characterization of a Cytokinin-Binding Protein from the Water-Soluble Fraction of Tobacco Leaves
スポンサーリンク
概要
- 論文の詳細を見る
Cyiokinin-binding protein (CBPI) was purified from the water soluble fraction of tobacco leaves by successive chromatography on benzyladenine-linked (BA-linked) Sepharose 4B, TSK-Gel G3000SWXL, t-zeatin-linked Sepharose 6B and TSK-Gel G3000SWXL. CBPI was obtained as a monomer with a molecular weight of 31 kDa. It has one cytokinin-binding site, which shows a high affinity for BA (Kd= 1.1×10^<-7> M) and other cyiokinins. Biologically active cyiokinins competed with BA for binding to this protein, while biologically inactive analogues of adenine did not. In all cases, cyiokinin-binding activity was assayed by equilibrium dialysis.
- 日本植物生理学会の論文
著者
-
Tsuji Hideo
Department of Botany, Faculty of Science, Kyoto University
-
Tsuji Hideo
Department Of Biology Kobe Women's University
-
Tsuji Hideo
Department Of Botany Faculty Of Science Kyoto University Kyoto
-
Momotani Eiki
Department Of Botany Faculty Of Science Kyoto University Kyoto:(present)national Institute For Basic
関連論文
- Induction of Enzymes Involved in the Ascorbate-Dependent Antioxidative System, Namely, Ascorbate Peroxidase, Monodehydroascorbate Reductase and Dehydroascorbate Reductase, after Exposure to Air of Rice (Oryza sativa) Seedlings Germinated under Water
- Early Light-Response of psaD, psaE and psaH Gene Families of Photosystem I in Nicotiana sylvestris : PSI-D has an Isoform of Very Quick Response : ENVIRONMENTAL AND STRESS RESPONSES : GENE STRUCTURE AND EXPRESSION
- Cytokinin Induces a Rapid Decrease in the Levels of mRNAs for Catalase, 3-Hydroxy-3-methylglutaryl CoA reductase, Lectin and Other Unidentified Proteins in Etiolated Cotyledons of Cucumber : ENVIRONMENTAL AND STRESS RESPONSES : GENE STRUCTURE AND EXPRESSI
- Effects of 5-Aminolevulinic Acid on the Accumulation of Chlorophyll b and Apoproteins of the Light-Harvesting Chlorophyll a/b-Protein Complex of Photosystem II
- Isolation and Characterization of a Cytokinin-Binding Protein from the Water-Soluble Fraction of Tobacco Leaves
- Resistance to Oxidative Injury in Submerged Rice Seedlings after Exposure to Air : ENVIRONMENTAL AND STRESS RESPONSES
- Mechanism of Benzyladenine-Induced Stimulation of the Synthesis of 5-Aminolevulinic Acid in Greening Cucumber Cotyledons:Benzyladenine Increases Levels of Plastid tRNA^ : GROWTH AND DEVELTOPMENT : PROTEINS, ENZYMES AND METABOLISM
- Development of the O_2^--Detoxification System during Adaptation to Air of Submerged Rice Seedlings : ENVIRONMENTAL AND STRESS RESPONSES : PROTEINS, ENZYMES AND METABOLISM
- Changes in Levels of Heme a, Protoheme and Protochlorophyll(ide) in Submerged Rice Seedlings after Exposure to Air : ENVIRONMENTAL AND STRESS RESPONSES : PROTEINS, ENZYMES AND METABOLISM