Photoactivation of Oxygen-Evolving Enzyme in Dark-Grown Pine Cotyledons: Relationship between Assembly of Photosystem II Proteins and Integration of Manganese and Calcium
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概要
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Dark-grown cotyledons of pine (Pinus thunbergii) did not exhibit O_2 evolution, but this capability was rapidly activated by illumination for a short period (photoactivation). To examine the biochemical changes which accompany the process of photoactivation in gymnosperms, a method enabling the preparation of highly active O_2-evolving photosystem II (PS II) membranes was applied to light-grown, dark-grown, and photoactivated cotyledons. PS II membranes prepared from light-grown cotyledons exhibited high O_2-evolving activity, and contained all the intrinsic proteins as well as the three extrinsic proteins (32, 23 and 17 kDa) associated with PS II. These membranes were also found to contain 4.4 Mn and 0.83 Ca/PS II reaction center. PS II membranes from dark-grown cotyledons contained all the intrinsic proteins, but preserved only 32 kDa extrinsic protein, and zero Mn and 0.85 Ca/PS II reaction center. The two extrinsic proteins (23 and 17 kDa) absent in the PS II membranes from dark-grown cotyledons were, however, present as mature forms in whole thylakoid membranes from the corresponding sample. The PS II membranes isolated from photoactivated cotyledons showed a high activity of O_2 evolution and retained the three extrinsic proteins, 5.3 Mn and 1.1 Ca/PS II reaction center, respectively. The results indicated that Mn and the two extrinsic proteins were tightly integrated in the O_2-evolving apparatus during the process of photoactivation but integration of Ca preceded the integration of Mn by photoactivation.
- 日本植物生理学会の論文
著者
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Inoue Yorinao
Solar Energy Research Group, Institute for Physical and Chemical Research
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SHINOHARA Kenji
Molecular and Cell Biology Section, Bio-Resources Technology Division, Forestry and Forest Products
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Ono Taka-aki
Solar Energy Research Group, The Institute of Physical and Chemical Research
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Ono Taka-aki
Solar Energy Res. Group The Inst. Phys.& Chem. Res (riken)
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Inoue Yorinao
Solar Energy Research Group Riken
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Shinohara Kenji
Molecular And Cell Biology Section Bio-resources Technology Division Forestry And Forest Products Re
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Shinohara Kenji
Molecular And Cell Biology Section Bio-resources Technology Division Forestry And Forest Products Re
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Inoue Yorinao
Solar Ener.res.group Riken
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