A New 4.8-kDa Polypeptide Intrinsic to the PS II Reaction Center, as Revealed by Modified SDS-PAGE with Improved Resolution of Low-Molecular-Weight Proteins

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概要

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• Low-molecular-weight polypeptides in various PS II preparations from spinach and wheat were analyzed by modified SDS-PAGE, which gave good resolution of low-molecular-weight proteins with minimized interference by lipids. PS II membrane fragments contained at least nine low-molecular-weight polypeptides of between 3.9 kDa and 11 kDa, and all of them were identified in thylakoid membranes. Of these nine polypeptides, the 10-kDa phosphoprotein, the 5-kDa, 4.8-kDa, and 4.1-kDa polypeptides, and the two subunits of cytochrome b_<559>S were commonly found in O_2-evolving core complexes of wheat and spinach. In contrast, PS II reaction center complexes that consisted of D1, D2 and two cytochrome b_<559>S polypeptides retained only the 4.8-kDa polypeptide. Analysis by Western blotting revealed that the 4.8-kDa polypeptide is an intrinsic component of the PS II reaction center.

• 日本植物生理学会の論文

著者

• Inoue Yorinao

  Solar Energy Research Group Riken

• Ikeuchi Masahiko

  Solar Energy Research Group The Institute Of Physical And Chemical Research (riken)

• Ikeuchi Masahiko

  Solar Energy Research Group Riken (the Institute Of Physical And Chemical Research)

• Inoue Yorinao

  Solar Ener.res.group Riken

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