Enhancement of the Division of Equisetum arvense Protoplasts in Culture by Activated Charcoal and Their Further Development

概要

論文の詳細を見る
Protoplasts were isolated from subcultured gametophytes of Equisetum arvense by treatment with Driselase and then cultured in vitro. Addition of activated charcoal (AC) to the culture medium enhanced the rate of cell division, as well as the survival of both protoplasts and regenerated protoplasts. However, subsequent division of cells was not observed after one or two cycles of replication in cultures supplemented with AC. When regenerated protoplasts were transferred to fresh medium without AC 3 to 5 weeks after the first plating, the transferred cells formed rhizoids and developed into small, young gametophytes without the prior formation of cell clusters or calluses. Furthermore, sprophytic shoots differentiated from the protoplast-derived gametophytes when they were cultured on medium supplemented with 6-benzylamino-purine (BA).
日本植物生理学会の論文