Functional Expression of Agrobacterium tumefaciens T-DNA onc-genes in Asparagus Crown Gall Tissues

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We analyzed Asparagus crown gall tissues transformed with A. tumefaciens C58 and C58Cl (pTiB6S3) and selected for hormone autotrophic growth. No increased IAA levels were observed in theAsparagustumor lines notwithstanding the presence of all three T-DNA onc genes. The endogenous cytokinin levels indicate that Asparagus crown gall is dependent on enhanced zeatin riboside equivalent levels for its growth. We conclude that phyiohormone autotrophic growth of Asparagus crown gall tissue seems only to be dependent upon an active gene 4, inducing enhanced cytokinin levels. Moreover, the presence of an active gene 1 seems to be lethal as was indicated by the absence of tryptophan-2-mono-oxygenase activity in transformed tissues and the toxicity of exogenously supplied indole-3-acetamide (IAM) or naphthalene-1-acetamide (NAM) as a substitute for an active gene 1.
日本植物生理学会の論文