Immunoaffinity Purification of Indole-3-acetamide Using Monoclonal Antibodies

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概要

• 論文の詳細を見る

• A single-step immunoaffinity purification procedure using monoclonal antibodies was developed to isolate indole-3-acetamide from plant extracts. Antibodies from a selected clone, raised against IAA-C1'-BSA, with pronounced ability to recognize indole-3-acetamide (IAM) were used to prepare an immunoaffinity absorbent. Antibodies purified by thiophilic interaction chromatography were immobilized on divinylsulfone-activated agarose. This column shows a very high selectivity towards IAM compared to IAA. This single step of immunoaffinity purification gave plant extracts of sufficient purity for direct quantification by on-line spectrofluorimetry after an analytical ion-suppression-HPLC run. Successive approximation by a second analytical ion-pairing-HPLC run confirmed the validity of this analytical technique.

• 日本植物生理学会の論文

著者

• Onckelen Henri

  Department Of Biology Uia Universiteitsplein

• Rudelsheim Patrick

  Department of Biology

• Ulvskov Peter

  Institute of Plant Physiology, Royal Veterinary and Agricultural University

• Marcussen Jan

  Institute of Plant Physiology, Royal Veterinary and Agricultural University

• Rajagopal Ranganatha

  Institute of Plant Physiology, Royal Veterinary and Agricultural University

• Prinsen Els

  Department of Biology, University of Antwerpen, UIA, Universiteitsplein

• Prinsen E

  Uia Wilrijk Bel

• Prinsen Els

  Department Of Biology University Of Antwerp (uia)

• Ulvskov Peter

  Institute Of Plant Physiology Royal Veterinary And Agricultural University:biotechnology Section De

• Marcussen Jan

  Institute Of Plant Physiology Royal Veterinary And Agricultural University

• Rajagopal R

  Royal Vet. & Agri. Univ. Frederiksberg Dnk

• Ruedelsheim P

  Univ. Antwerpen Wilrijk Bel

• Onckelen Henri

  Department Of Biology University Of Antwerpen Uia Universiteitsplein

関連論文

• Kinetics of Endogenous Cytokinin, IAA and ABA Levels in Relation to the Growth and Morphology of Tobacco Crown Gall Tissue
• Functional Expression of Agrobacterium tumefaciens T-DNA onc-genes in Asparagus Crown Gall Tissues
• Immunoaffinity Purification of Indole-3-acetamide Using Monoclonal Antibodies
• The Effect of Mutations in the T-DNA Encoded Auxin Pathway on the Endogenous Phytohormone Content in Cloned Nicotiana tabacum Crown Gall Tissues
• Dynamics of Endogenous IAA and Cytokinins during the Growth Cycle of Soybean Crown Gall and Untransformed Callus
• Effect of Heat Stress on Photosynthetic Activity and Chloroplast Ultrastructure in Correlation with Endogenous Cytokinin Concentration in Maize Seedlings
• Functional Correlation between Endogenous Phytohormone Levels and Hormone Autotrophy of Transformed and Habituated Soybean Cell Lines
• Extraction and quantitative analysis of l-aminocyclopropane-l-carboxylic acid in plant tissue by gas chromatography coupled to mass spectrometry

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