The Effect of Mutations in the T-DNA Encoded Auxin Pathway on the Endogenous Phytohormone Content in Cloned Nicotiana tabacum Crown Gall Tissues
スポンサーリンク
概要
- 論文の詳細を見る
We analyzed the endogenous auxin and cytokinin levels of cloned Nicotiana tabacum SR1-lines induced either by the wild-type Agrobacterium tumefaciens C58 strain or by mutants affected in the T-DNA-encoded IAA biosynthesis pathway. The wild-type SR1-C58 line contained up to 20 times more IAA than a nontransformed SR1-callus line. The mutant lines affected in gene 1 (iaaM^-) or gene 2 (iaaH^-) contained intermediate levels of IAA. Analysis of the endogenous levels of indole-3-acetamide (IAM) in the nontransformed SRI callus line, the wild-type SR1-C58 and the two mutant lines confirmed the T-DNA-induced IAA biosynthesis pathway in the transformed tumor cells. Supplementing auxin to the mutant lines resulted in complete suppression of the shoot-forming ability, but no changes in the endogenous IAA Ievels. There was no marked difference in the cytokinin level between the non-transformed callus line and the wild type tumor line. The two mutant lines, however, showed a 20- to 30-fold higher cytokinin level which was not affected by the addition of NAA. The T-DNA encoded hormone biosynthetic pathways are discussed in relation to pathways of the host plant.
- 日本植物生理学会の論文
著者
-
Inze Dirk
Laboratorium Voor Genetica Departement Genetica Vlaams Interuniversitair Instituut Voor Biotechnolog
-
Inze Dirk
Laboratorium Voor Genetica Rijksuniversiteit Gent
-
Onckelen Henri
Department Of Biology Uia Universiteitsplein
-
Rudelsheim Patrick
Department of Biology
-
Greef Jan
Department of Biology
-
Prinsen Els
Department of Biology, University of Antwerpen, UIA, Universiteitsplein
-
Lijsebettens Maria
Laboratorium voor Genetica, Rijksuniversiteit Gent
-
Montagu ark
Laboratorium voor Genetica, Rijksuniversiteit Gent
-
Greef Jan
Department Of Biology Uia Universiteitsplein
-
Prinsen E
Uia Wilrijk Bel
-
Prinsen Els
Department Of Biology University Of Antwerp (uia)
-
Rudelsheim Patrick
Department Of Biology University Of Antwerpen
-
Montagu Ark
Laboratorium Voor Genetica Rijksuniversiteit Gent
-
Lijsebettens Maria
Laboratorium Voor Genetica Rijksuniversiteit Gent
-
Onckelen Henri
Department Of Biology University Of Antwerpen Uia Universiteitsplein
関連論文
- Kinetics of Endogenous Cytokinin, IAA and ABA Levels in Relation to the Growth and Morphology of Tobacco Crown Gall Tissue
- Functional Expression of Agrobacterium tumefaciens T-DNA onc-genes in Asparagus Crown Gall Tissues
- Immunoaffinity Purification of Indole-3-acetamide Using Monoclonal Antibodies
- The Effect of Mutations in the T-DNA Encoded Auxin Pathway on the Endogenous Phytohormone Content in Cloned Nicotiana tabacum Crown Gall Tissues
- Dynamics of Endogenous IAA and Cytokinins during the Growth Cycle of Soybean Crown Gall and Untransformed Callus
- Effect of Heat Stress on Photosynthetic Activity and Chloroplast Ultrastructure in Correlation with Endogenous Cytokinin Concentration in Maize Seedlings
- The NADPH : Quinone Oxidoreductase P1-ζ-crystallin in Arabidopsis Catalyzes the α, β-Hydrogenation of 2-Alkenals : Detoxication of the Lipid Peroxide-Derived Reactive Aldehdes
- ENZYMIC CHARACTERS AND OVEREXPRESSION IN TOBACCO OF Arabidopsis P1/Z-CRYSTALLIN
- PLANT ζ-CRYSTALLIN (Arabidopsis P1 PROTEIN) IS A NADPH-DIAMIDE REDUCTASE
- Herbicide Safener-Inducible Gene Expression in Arabidopsis thaliana