Purification and Partial Characterization of a Lectin from the Bark of Japanese Elderberry (Sambucus sieboldiana)
スポンサーリンク
概要
- 論文の詳細を見る
A lectin [Sambucus sieboldiana agglutinin (SSA)] was purified from the twigs of Sambucus sieboldiana by repeated affinity chromatography on fetuin-Sepharose. SSA had a molecular weight (Mr) of approximately 160 K on gel filtration and consisted of two types of subunit of which the molecular weights ranged from 31 to 37 K. SSA agglutinated human erythrocytes irrespective of their blood type and the hemagglutination was inhibited by a very low concentration of Neu5Ac(a2-6)lactose, suggesting that SSA has a carbohydrate-binding specificity similar to that of the lectin previously isolated from the bark of S. nigra (SNA). However, the Ouchterlony double-diffusion analysis of these lectins with an antibody raised against SSA showed that SSA was immunologically related but not identical to SNA.
- 日本植物生理学会の論文
著者
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Shibuya Naoto
National Food Research Institute Ministry Of Agriculture
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Tazaki Kiyoshi
Forestry And Forest Products Research Institute Ministry Of Agriculture
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Tazaki Kiyoshi
Forestry And Forest Product Res. Inst.
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Shibuya Naoto
National Food Research Institute
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