The Bark Lectin of Robinia pseudoacacia: Purification and Partial Characterization
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概要
- 論文の詳細を見る
lectin was purified from the bark of Robinia pseudoacacia by sequential ion-exchange chromatography on DEAE-Sepharose and CM-Toyopearl. The purified lectin was estimated to have a molecular weight of 106 kDa and to be a homotetramer of subunits with a molecular weight of 29 kDa. Antibodies raised against the bark lectin cross-reacted with a 29-kDa polypeptide during Western blot analysis, showing that the antibodies are specific for the bark lectin. The anti-bodies against the lectin from Robinia bark cross-reacted with polypeptides in extracts of the seeds and bark of Sophora japonica, indicating that the lectin from Robinia bark is immunologically related to the lectins of Sophora. However, the antibodies did not cross-react with proteins from Robinia seeds and leaves. The first twenty amino acid residues from the N-terminus of the lectin from Robinia bark were determined and compared with those of the Sophora lectins.
- 日本植物生理学会の論文
著者
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Tazaki Kiyoshi
Forestry And Forest Products Research Institute Ministry Of Agriculture Forestry And Fisheries
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Tazaki Kiyoshi
Forestry And Forest Product Res. Inst.
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Yoshida Kazumasa
Forestry And Forest Products Research Institute
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Yoshida Kazumasa
Forestry And Forest Products Research Institute Ministry Of Agriculture Forestry And Fisheries
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