A Modified Micro-Drop Bioassay Using Dwarf Rice for Detection of Femtomol Quantities of Gibberellins

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概要

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• The sensitivity of the micro-drop assay with dwarf rice (Oryza sativa L., cv. Tan-ginbozu and cv. Waito-C) to gibberellins (GAs) was increased conspicuously by the use of assay plants that has been treated with uniconazole (S-3307), an inhibitor of the biosynthesis of GAs. The Tan-ginbozu plants treated with S-3307 responded to 10 fmol/plant of GA_3 (ca. 3.5 pg/plant) and to 30 fmol/plant of gibberellins A_1, A_4, A_7, A_<19> and A_<20>. Waito-C plants treated with S-3307 responded to 10 fmol of GA_3 and to 30 fmol/plant of gibberellins A_1, A_4 and A_7. Gibberellins A_9, A_<19> and A_<20> had much less of an effect on the treated Waito-C plants than did gibberellins A_1, A_3, A_4 and A_7. Furthermore, treatment with S-3307 counteracted the inhibition of growth of both cultivars by abscisic acid. Thus, the modified micro-drop assay should prove very useful for the detection of minute amounts of GAs in plant extracts.

• 日本植物生理学会の論文

著者

• NISHIJIMA Takaaki

  Department of Applied Physiology, National Research Institute of Vegetables, Ornamental Plants and T

• KATSURA Naoki

  Department of Applied Physiology, National Research Institute of Vegetables, Ornamental Plants and T

• Katsura Naoki

  Department Of Applied Physiology National Research Institute Of Vegetables

• Nishijima Takaaki

  Department Of Applied Physiology National Research Institute Of Vegetables

関連論文

• Endogenous Gibberellins in Mature Seed of Raphanus sativus L. cv. Taibyo-sobutori(Organic Chemistry)
• Qualitative and Quantitative Analysis of Endogenous Gibberellins in Raphanus sativus L. during Cold Treatment and the Subsequent Growth
• A Modified Micro-Drop Bioassay Using Dwarf Rice for Detection of Femtomol Quantities of Gibberellins

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