Molecular PropertieS Of the ATP:_D-Fructose-6-Phosphate 1-Phosphotransferase Isoenzymes from Cucumis sativus
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概要
- 論文の詳細を見る
The ATP:D-fructose-6-phosphate 1-phosphotransferase (EC 2.7.1.11) isoenzymes from cucumber seeds were separated and purified. The calculated molecular weights of the two isoenzymes (approximately 180,000) are similar and the isoenzymes are probably hetro-tetramers. The purified isoenzymes contained three polypeptides of 53.3, 41.5 and 39.0 kDa for the plastid and 47.2,42.4 and 40.4 for the cytosolic isoenzyme, respectively. The purified phosphofructokinase isoenzymes were used as the antigen for the production of polyclonal antibodies in rabbits. The obtained antisera clearly indicated that there is no immunological similarity between the two isoenzymes. The results also show that the phosphofructokinase isoenzymes in cucumber are not merely different stages of association of the same protein.
- 日本植物生理学会の論文
著者
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Botha Frederik
Department Of Botany And Genetics University Of The Orange Free State
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Botha Frederik
Department Of Botany University Of The Orange Free State
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Small J
Department Of Botany University Of The Orange Free State
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Cawood Marieta
Department of Botany, University of the Orange Free State
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Small J.
Department of Botany, University of the Orange Free State
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Cawood Marieta
Department Of Botany University Of The Orange Free State
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Small J.
Department Of Botany University Of The Orange Free State
関連論文
- Effect of Anoxia on the Expression and Molecular Form of the Pyrophosphate Dependent Phosphofructokinase
- Immunological Characterization of the Pyrophosphate Dependent Fructose- 6-Phosphate Phosphotransferase
- Molecular PropertieS Of the ATP:_D-Fructose-6-Phosphate 1-Phosphotransferase Isoenzymes from Cucumis sativus
- Activity of Key Enzymes Involved in Carbohydrate Metabolism in Phaseolus vulgaris Cell Suspension Cultures
- Isolation and Characterization of Pyrophosphate : _D-Fructose-6-phosphate 1-Phosphotransferase from Cucumber Seeds
- Kinetic Propertles of the ATP-Dependent Phosphofructoklnase Isoenzymes from Cucumber Seeds