Characterization of Plastocyanin Isolated From Brazilian Elodea
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概要
- 論文の詳細を見る
Plastocyanin obtained from an aquatic higher plant, Brazilian elodea, was characterized by electronic absorption, CD, and EPR spectroscopy. The blue copper center of Brazilian elodea plastocyanin is electronically and geometrically similar to that of terrestrial higher-plant (cucumber, spinach, poplar, etc.) and algal plastocyanins as well as that of another aquatic higher-plant (hornwort) plastocyanin. The midpoint redox potential of the copper in the plastocyanin was estimated to be +364 mV at pH 7.2, which is in the range of midpoint potentials from +347 to + 395 mV reported for plastocyanins. The ratio of Phe/Tyr residue was determined to be 3 by amino acid analysis. Values of 2-3.5 have been commonly observed in many higher-plant plastocyanins; a conspicuous exception to this is hornwort plastocyanin with a Phe/Tyr value of 9.
- 日本植物生理学会の論文
著者
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Suzuki Shinnichiro
Institute Of Chemistry College Of General Education Osaka University
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Sakurai Takeshi
Institute of Chemistry, College of General Education, Osaka University
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Nakajima Takuo
Lake Biwa Research Institute
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Nakajima Takuo
Lake Biwa Institute
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Sakurai Takeshi
Institute Of Chemistry College Of General Education Osaka University
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Nakajima T
Lake Biwa Res. Inst.
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Sakurai Takeshi
Institute For Molecular Science
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