Nitrogen-Regulated Accumulation of mRNA and Protein for Photosynthetic Carbon Assimilating Enzymes in Maize

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Nitrogen and light effects on kinetic patterns of mRNA and protein accumulations for ribulose 1,5-bisphosphate carboxylase (RuBPC), phosphoenolpyruvate carboxylase (PEPC), and pyruvate orthophosphate dikinase (PPDK) were examined by analyzing the transient states induced by supplementing nitrogen or light intensity to Zea mays plants grown for 3 weeks under low nitrate (0.8 mM KNO_3) and/or low light (12% full sunlight) conditions. When plants grown previously under low nitrate were supplemented with high nitrate (16 mM KNO_3) for 3 days under high sunlight, mRNAs for these enzymes accumulated progressively as did their proteins. The accumulation of mRNA for these enzymes was stimulated in plants previously grown under high nitrate by increasing light intensity from low light to high light (48% full sunlight). This stimulation, however, did not occur in plants previously grown under low nitrate. These results imply that nitrogen is a regulatory factor essential for the light stimulation of mRNA accumulation of these enzymes. The kinetic patterns of protein and mRNA accumulation also indicate that the accumulation of proteins of PEPC and PPDK is a reflect of the level of respective mRNA, irrespective of the conditions used for inducing transient changes in gene expression. For RuBPC, protein and mRNA accumulations did not necessarily coincide.
日本植物生理学会の論文