Characterization of the mRNA for the α-Amylase from Wheat

スポンサーリンク

概要

• 論文の詳細を見る

• Characterization of the mRNA for the α-amylase from wheat was made by fractionation of the total membrane-bound polysomal RNA and by immunoprecipitation of in vitro synthesized α-amylase by its specific antibody. The content of the mRNA for the α-amylase in the membrane-bound polysomal fraction from germinating wheat seeds was estimated as 5-10% of the total mRNA in this fraction. RNA, separated in high resolution on acid-urea-agarose gels by electrophoresis, was recovered from the gels by a new method. Its translation products in rabbit reticulocyte lysates were analyzed with a specific antibody against α-amylase. The size of the mRNA for α-amylase was estimated as 5.0×10^5 daltons.

• 日本植物生理学会の論文

著者

• Motojima Kiyoto

  Laboratory Of Microbiological Chemistry Mitsubishi-kasei Institute Of Life Sciences

• Sakaguchi Kenji

  Laboratory Of Microbiological Chemistry Mitsubishi-kasei Institute Of Life Sciences

関連論文

• Characterization of the mRNA for the α-Amylase from Wheat
• Part of the Lysyl Residues in Wheat α-Amylase is Methylated as N-ε-Trimethyl Lysine

スポンサーリンク