An Improved Method for Determining the Ionic Conductance and Capacitance of the Membrane of Chara corallina
スポンサーリンク
概要
- 論文の詳細を見る
The assumption of a single exponential change for the voltage response caused by a square current of subthreshold intensity is not necessarily an adequate explanation of the situation in the Chara membrane. By improving the speed of electronics for current clamping and the accuracy of data simulation, it was found that an additional process, which can be generally simulated with another exponential function, was superimposed on the voltage response. Such an additional exponential process may be partly atributed to the response of the tonoplast, if the internal potential measuring microelectrcde is in the vacuole. However, this additional process still remained in the voltage response of the plasmalemma of the Chara internode when the tonoplast and a large amount of endoplasm were removed by internal perfusion with an glycol ether diaminetetraacetic acid solution. The additional exponential component arose from the change of the electromotive force of the single membrane during application of the test current pulse. The increase of the conductance of the plasmalemma alone was large during excitation, while that of the elecrostatic capacitance was negligible. On the other hand, the conductance and capacitance which reflect an additional exponential ionic process during the test pulse both increased greatly around the peak of excitation. This rise is the main reason for an apparent large increase of membrane capacitance which was deduced from a simple approximation of the membrane with a single time constant model.
- 日本植物生理学会の論文
著者
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Kami-ike Nobunori
Department Of Bioengineering Faculty Of Engineering Science Osaka University
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TAKEUCHI Yuko
Department of Dermatology, Gunma University School of Medicine
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Kishimoto Uichiro
Department of Biology, College of General Education, Osaka University
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Ohkawa Taka-aki
Department of Biology, College of General Education, Osaka University
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Takeuchi Yuko
Department Of Biology College Of General Education Osaka University
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Ohkawa Taka-aki
Department Of Biological Sciences Faculty Of Life And Environmental Science Shimane University
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Kishimoto Uichiro
Department Of Biology College Of General Education Osaka University
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