Plasmid DNA-induced Transformability of Yeast Cells during Spheroplast Conversion
スポンサーリンク
概要
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Changes in transformability, regenerability, cell fusibility and endocytotic activity of Saccharomyces cerevisiae yeast during spheroplast conversion were investigated using the cell wall digesting enxyme Xymolylase. The transformability with plasmid DNA, cell fusibility and endocytotic activity all reached maximum at the critical spheroplast conversion period when about half of the cells had been converted into spheroplasts. Lysosomotropic drugs, chloroquine and dansylcadaverine enhanced transformation and endocytosis two- to threefold. This study showed that a close relationship exists among transformability, cell fusing activity and endocytotic activity of spheroplasts.
- 日本植物生理学会の論文
著者
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Takagi Keiko
Biological Institute Faculty Of Science Nagoya University
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Yoshida Kazuo
Biological Institute Faculty Of Science Nagoya University
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