Cytological studies on mitochondria-induced cytoplasmic transformation in yeasts
スポンサーリンク
概要
- 論文の詳細を見る
In Saccharomyces cerevisiae, protoplasts from respiratory-deficient (rho^-) cytoplasmic mutant cells were transformed into respiratory-sufficient (rho^+) cells by incubation with mitochondria prepared from rho^+ cells in the presence of polyethylene glycol and CaCl_2. Mitochondria prepared from different species, Hansenula wingei and Schizo-saccharomyces pombe, also caused the transformation of S. cerevisiae rho^- protoplasts into the rho^+ cells as previously reported (14). The obtained transformants were confirmed to contain one nucleus and several mitochondrial DNAs by fluorescent staining of DNA. The transformants clearly restored cytochromes a and b while untransformed recipient cells lacked the cytochromes. In order to know the mechanism of the transformation, physiological measurement of endocytotic activity of protoplasts and cytological examination of mitochondria-protoplast aggregates under the transforming condition were performed. Protoplasts had significant endocytotic activity under this condition. On the other hand, fluorescence and electron microscopic observations indicated that mitochondria forming aggregates with protoplasts were subsequently integrated into recipient protoplasts through fusion rather than endocytosis. However, the possibility of endocytosis could not be completely excluded when the low frequency of the transformation (about 10^<-6> to 10^<-7>) was taken into account. This is discussed in this paper. A new convenient method for measuring endocytosis is also presented.
- 日本植物生理学会の論文
著者
-
Yoshida Kazuo
Biological Institute Faculty Of Science Nagoya University
-
Takeuchi.K Ikuo
Institute for Developmental Research, Aichi Prefectural Colony
-
Takeuchi.k Ikuo
Institute For Developmental Research Aichi Prefectural Colony
関連論文
- Physiological Effects of a and α Sexual Agglutination Substances on Mating Reaction in the Yeast Saccharomyces cerevisiae
- Effect of concanavalin A on the mating reaction in Saccharomyces cerevisiae
- Specific interaction of cytokinin binding protein with 40S ribosomal subunits in the presence of cytokinin in vitro
- Remarkable retardation of the senecence of tobacco leaf disks by cordycepin, an inhibitor of RNA polyadenylation
- Intra- and intergeneric mating behaviour of ascosporogenous yeasts I. Quantitative analysis of sexual agglutination
- Cytological studies on mitochondria-induced cytoplasmic transformation in yeasts
- Plasmid DNA-induced Transformability of Yeast Cells during Spheroplast Conversion
- RNA containing poly-A sequence in Pharbitis nil cotyledons and its template activity in vitro
- Interspecific and intraspecifc mitochondria-induced cytoplasmic transformation in yeasts
- Stepwise Transformation in Saccharomyces cerevisiae Yeast : Construction of Strains for Transformation and Subsequent Cytoductive Transfer of Plasmid DNA with Mitochondria
- Novel lectins in the endoplasmic reticulum of wheat germ and their possible role