Effect of concanavalin A on the mating reaction in Saccharomyces cerevisiae

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The effect of concanavalin A (Con A) on the process of mass mating of Saccharomyces cerevisiae was studied. Sexual agglutination was repressed by Con A at concentrations of 400 μg/ml and 500 μg/ml, but zygote formation was little affected at these concentrations. The action of Con A was antagonized specifically by α-methyl-D-mannoside. We compared the mode of inhibitory action on sexual agglutination of Con A with that of agglutination substances, cell surface glycoproteins responsible for sexual agglutination. The agglutination substances inhibited the formation of small cell aggregates consisting of less than fifty cells thought to be necessary for the formation of zygotes. Con A, on the contrary did not inhibit the formation of small aggregates, but inhibited the formation of large cell aggregates consisting of more than hundred cells by interfering with the fusion of small cell aggregates. Univalent Con A inhibited isoagglutination caused by high concentrations of native Con A. Specific. binding of Con A to the cell surface was observed by using fluorescent Con A. A procedure to prepare univalent Con A using Enzygel, a trypsin-Sepharose conjugant is described.
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