Messenger and ribosomal RNA hydrolysis by ribonucleases II. : Changes in ribonuclease activities and ribosomes of barley leaves during the early stages of powdery mildew infection

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概要

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• We have monitored changes in the properties of two barley (Hordeum vulgare L.) leaf RNases with respect to their action on polysomal messenger RNA (mRNA) and the RNA of isolated ribosomes during the early stages of infection by the powdery mildew fungus (Erysiphe graminis f. sp. hordei). The results presented support the following conclusions. (i) At 48 hr after inoculation, the pH 5 insoluble RNase undergoes significant changes in its catalytic properties. This is evident from the finding that under limit digestion conditions, the enzyme from inoculated leaves hydrolyzes chloroplast polysomal mRNA and produces far greater quantities of chloroplast monosomes than does the corresponding enzyme from healthy leaves. (ii) The acid soluble oligonucleotide fragments produced by the soluble RNase from healthy and inoculated leaves (at 48 hr after inoculation) in the RNA of isolated ribosomes are quantitatively significantly different. This suggests a change in the properties of the soluble RNase during the initial stages of host-parasite interactions. (iii) As early as 24 hr after inoculation, there is a dramatic change in the distribution of the pH 5 insoluble and soluble RNase cleavage sites in the RNA of ribosomes indicating a readily detectable conformational change in the ribonucleoprotein particles. (iv) These changes in the RNases and ribosomes are only detectable in the susceptible cultivars of barley and not in a cultivar which is genetically resistant to race 3 of the powdery mildew fungus.

• 日本植物生理学会の論文

著者

• Chakravorty Arun

  Laboratory Of Plant Biochemistry And Molecular Pathology Department Of Biochemistry University Of Qu

• Simpson Robert

  Laboratory Of Plant Biochemistry And Molecular Pathology Department Of Biochemistry University Of Qu

• Scott K.J.

  Laboratory of Plant Biochemistry and Molecular Pathology, Department of Biochemistry, University of

• Scott K.j.

  Laboratory Of Plant Biochemistry And Molecular Pathology Department Of Biochemistry University Of Qu

関連論文

• Messenger and ribosomal RNA hydrolysis by ribonucleases II. : Changes in ribonuclease activities and ribosomes of barley leaves during the early stages of powdery mildew infection
• Messenger and ribosomal RNA hydrolysis by ribonucleases I. The action of two barley leaf ribonucleases on the messenger and ribosomal .RNA of isolated polysomes
• Protein synthesis by polysomes isolated from wheat leaves

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