Messenger and ribosomal RNA hydrolysis by ribonucleases I. The action of two barley leaf ribonucleases on the messenger and ribosomal .RNA of isolated polysomes

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概要

• 論文の詳細を見る

• When barley (Hordeum vulgare L.) leaf polysomes are incubated with two RNase fractions (the pH 5 insoluble and soluble RNases) under limit digestion conditions, the two enzymes exhibit characteristic preference for messenger and ribosomal RNA (mRNA and rRNA) hydrolysis. The pH 5 insoluble RNase from a cultivar of barley, Prior, and the corresponding enzyme from two near-isogenic lines (M1622 and M1623) cleave polysomal mRNA at specific sites and generate polysome profiles that are unique to the cultivar. By contrast, the soluble RNase from barley leaves, although a typical endoribonuclease, catalyzes no detectable hydrolysis of polysomal mRNA. Both of these barley leaf RNases hydrolyze rRNA when either polysomes or monosomes are treated with these enzymes. With polysomes as substrate, the pH 5 insoluble RNase hydrolyzes the high molecular weight RNA component of both large and small subunits of chloroplast and cytoplasmic ribosomes. The soluble RNase preferentially hydrolyzes the high molecular weight RNA component of the small subunit of chloroplast and cytoplasmic ribosomes. Analytical gel electrophoresis of the RNA of the RNase-treated monosomes has revealed that both enzymes hydrolyze rRNA into very small fragments. However, despite scission in rRNA at multiple sites, the RNase-treated monosomes remain active in polyuridylate-directed polyphenylalanine synthesis.

• 日本植物生理学会の論文

著者

• Chakravorty Arun

  Laboratory Of Plant Biochemistry And Molecular Pathology Department Of Biochemistry University Of Qu

• Simpson Robert

  Laboratory Of Plant Biochemistry And Molecular Pathology Department Of Biochemistry University Of Qu

• Scott K.J.

  Laboratory of Plant Biochemistry and Molecular Pathology, Department of Biochemistry, University of

• Scott K.j.

  Laboratory Of Plant Biochemistry And Molecular Pathology Department Of Biochemistry University Of Qu

関連論文

• Messenger and ribosomal RNA hydrolysis by ribonucleases II. : Changes in ribonuclease activities and ribosomes of barley leaves during the early stages of powdery mildew infection
• Messenger and ribosomal RNA hydrolysis by ribonucleases I. The action of two barley leaf ribonucleases on the messenger and ribosomal .RNA of isolated polysomes
• Protein synthesis by polysomes isolated from wheat leaves

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