Protein synthesis by polysomes isolated from wheat leaves

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概要

• 論文の詳細を見る

• When polysomes isolated from wheat (Triticum vulgare L., var. W2691) leaves are incubated with the soluble components of wheat germ protein synthesizing system (S176 fraction), the in vivo initiated polypeptide chains are elongated and various size classes of polypeptides are radiolabelled. Under these conditions, there is little or no reinitiation of polypeptides by ribosomes that have completed the elongation of in vivo initiated chains. The time course of labelling and the large size of the polypeptides synthesized suggest that most of the polypeptides are elongated to completion in the cell-free system. The size classes of newly synthesized polypeptides thus reflect the size classes of wheat leaf polysomal mRNA populations. In the protein synthesizing system reconstituted with wheat leaf polysomes and S 176 fraction from wheat germ, cycloheximide inhibits the elongation of certain size classes of polypeptides. At high concentrations (5 × 10^<-3> M and greater), it completely abolishes the translation of all size classes of polysomal mRNA. Chloramphenicol, on the other hand, preferentially inhibits the synthesis of certain classes of polypeptides including those that correspond in size to pplypeptides known to be synthesized by isolated chloroplasts. These characteristics of cell-free translation suggest that analytical gel electrophoresis of the products of polysomal mRNA translation represents a potentially powerful biochemical tool for monitoring mRNA populations in plant tissues.

• 日本植物生理学会の論文

著者

• Chakravorty Arun

  Laboratory Of Plant Biochemistry And Molecular Pathology Department Of Biochemistry University Of Qu

• Scott K.j.

  Laboratory Of Plant Biochemistry And Molecular Pathology Department Of Biochemistry University Of Qu

• Scott K.j.

  Laboratory Of Plant Biochemistry And Molecular Pathology Department Of Biochemistry University Of Qu

• Chakravorty Arun

  Laboratory Of Plant Biochemistry And Molecular Pathology Department Of Biochemistry University Of Qu

• Musk Peter

  Laboratory of Plant Biochemistry and Molecular Pathology, Department of Biochemistry, University of

• Musk Peter

  Laboratory Of Plant Biochemistry And Molecular Pathology Department Of Biochemistry University Of Qu

関連論文

• Messenger and ribosomal RNA hydrolysis by ribonucleases II. : Changes in ribonuclease activities and ribosomes of barley leaves during the early stages of powdery mildew infection
• Messenger and ribosomal RNA hydrolysis by ribonucleases I. The action of two barley leaf ribonucleases on the messenger and ribosomal .RNA of isolated polysomes
• Protein synthesis by polysomes isolated from wheat leaves

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