Light-reactivation of (Tris-washed)-DPIP-treated chloroplasts : manganese incorporation, chlorophyll fluorescence, action spectrum and oxygen evolution
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概要
- 論文の詳細を見る
Incorporation of ^<54>Mn into Tris (pH 8.8, 1 hr)-washed chloroplasts and oxygen evolving activity were stimulated by DPIP treatment and light-reactivation, but inhibited by atebrin, DCCD, gramicidin J, DCMU and EDTA. Omission of DTT on the light-reactivation resulted in accumulation or deposit of Mn in chloroplasts which had no function for the recovery of oxygen-evolving activity. The chlorophyll fluorescence quenched by Tris-washing was restored by DPIP-treatment and light-reactivation. The chloroplast structure, monitored by its packed volume and optical density at 750 nm, changed reversibly with inhibition and DPIP-treatment. The action spectrum of light-reactivation suggested that the effective light-receptor might be photosystem II.
- 日本植物生理学会の論文
著者
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Yamashita Takashi
Faculty Of Science And Technology Tokyo University Of Science
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Tomita Giiti
Faculty Of Agriculture Kyushu University
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Yamashita Takashi
Faculty Of Agriculture Kyushu University:(present)department Of Biological Science Tsukuba Universit
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Yamashita Takashi
Faculty Of Agriculture Kyushu University
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