Effects of urea and o-phenanthroline on F-695 emission in chloroplasts

概要

論文の詳細を見る
1. To clarify the nature of the chlorophyll species which fluoresces at about 695 nm in vivo (F-695), effects of the addition of urea and related compounds and of urea plus o-phenanthroline on the emission spectra of spinach chloroplast fragments, at 77°K, were examined. 2. F-695 emission was partially decreased by the presence of a low concentration (0.1 M) of urea, thiourea, guanidine hydrochloride, methylguanidine hydrochloride, acetamide, N-methylurea, or dimethylurea. 3. The concurrent addition of o-phenanthroline with the reagents (0.1-1 M) caused a marked increase in F-695 emission and a decrease in F-685 emission. Methyl-substituted ureas and acetamide, however, were less effective. The effect was largely dependent on the pH of the sample. 4. These two effects, the decrease and increase in F-695 emission, were reversible and were inhibited by the presence of ethanol. 5. The phenomena are probably due to specific interactions between added reagents and the component(s) in chloroplasts which have an intimate connection with a postulated energy trap ; an in vivo species of chlorophyll responsible for the fluorescence emission near 695 nm.
日本植物生理学会の論文