Glutamine synthesis in germinating seeds of Cucurbita moschata

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概要

• 論文の詳細を見る

• During germination, an increase in glutamine and glutamine synthetase [L-glutamate: ammonia ligase (ADP), EC 6.3.1.2] occurred in the cotyledons reaching a maximum at 4 to 6 days. The enzyme had a Km of 4.5 mM for L-glutamate, and 0.67 mM for hydroxylamine. Hydroxylamine exhibited substrate inhibition kinetics. The enzyme was inhibited by calcium ion, fluoride ion and p-hydroxymercuribenzoate but not by EDTA, sodium ion or chloride ion. The sulfhydryl inhibition was reversed by dithiothreitol. In vivo synthesis of glutamine-14C from glutamate-^<14>C was found to parallel the increase in glutamine content and the in vitro glutamine synthetase activity during germination.

• 日本植物生理学会の論文

著者

• Lignowski Edward

  Department Of Horticulture University Of Illinois At Urbana-champaign

• SPLITTSTOESSER WALTER

  Department of Horticulture, University of Illinois at Urbana-Champaign

• CHOU KUO-HOWERE

  Department of Horticulture, University of Illinois at Urbana-Champaign

• Chou Kuo-howere

  Department Of Horticulture University Of Illinois At Urbana-champaign

• Splittstoesser Walter

  Department Of Horticuhure University Of Lllinois

• Splittstoesser Walter

  Department Of Horticulture University Of Illinois At Urbana-champaign

関連論文

• Glutamine synthesis in germinating seeds of Cucurbita moschata
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• GLUTAMATE UTILIZATION BY MAIZE SCUTELLUM
• Characterization of aspartate aminotransferase in germinating pumpkin cotyledons
• Alanine aminotransferase from Cucurbita moschata cotyledons
• The solubilization and degradation of pumpkin seed globulin during germination

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