Characterization of aspartate aminotransferase in germinating pumpkin cotyledons

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概要

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• During germination an increase in aspartate aminotransferase (E.C.2.6.1.1.) occurs in the cotyledons of pumpkin seedlings grown either in the light or dark with the bulk of the enzyme activity in the soluble fraction. The soluble and particulate enzymes had a pH optimum of 8.0, an α-ketoglutarate optimum of 0.02m_M and a broad aspartate optimum from 0.05 to 0.2m_M. The velocity of the reaction was proportional to the enzyme concentration over a wide range. The soluble enzyme was shown to require manganese and pyridoxal-5-phosphate and an active sulfhydryl group for maximum activitiy. The soluble enzyme was easily destroyed by pepsin while the particulate enzyme lost activity upon washing. It is suggested that aspartate aminotransferase plays a role in nitrogen metabolism during protein hydrolysis in germinating pumpkin cotyledons.

• 日本植物生理学会の論文

著者

• Splittstoesser Walter

  Department Of Horticulture University Of Illinois

• Splittstoesser Walter

  Department Of Horticuhure University Of Lllinois

関連論文

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• GLUTAMATE UTILIZATION BY MAIZE SCUTELLUM
• Characterization of aspartate aminotransferase in germinating pumpkin cotyledons
• Alanine aminotransferase from Cucurbita moschata cotyledons
• The solubilization and degradation of pumpkin seed globulin during germination

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