Role of lipid in the cellulose synthetase enzyme system from oat seedlings

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概要

• 論文の詳細を見る

• Particulate fractions making cellulose from UDP glucose (glucose-^<14>C) were obtained by chromatography of oat seedling cell wall-free homogenates on Sepharose 4B. All particle fractions obtained formed varying proportions of glucolipid and polysaccharide. The optimal pH for cellulose synthesis was about 8. Activity was higher in Tris buffer than in phosphate buffer. Dithiothreitol enhanced the cellulose synthetase activity. Glycerol (0.37M) in the incubation medium had no effect on either glycolipid or polysaccharide synthesis. Treatment of particles with phospholipase A (EC 3.1.1.4) inhibited the enzyme systems in some fractions obtained by Sepharose chromatography and increased them in others. Nan-ionic and anionic detergents greatly inhibited the enzymes. Addition of lecithin to detergent-treated particles partially restored enzyme activity. Solubilization of the enzyme with retention of activity was not obtained.

• 日本植物生理学会の論文

著者

• Pinsky A.

  Department Of Biochemistry And Statewide Air Pollution Research Center University Of California Rive

• Ordin L.

  Department of Biochemistry, University of California

• Ordin L.

  Department Of Biochemistry And Statewide Air Pollution Research Center University Of California Riverside

関連論文

• The effects of growth Inhibitors on xylan synthetase activity in oat coleoptiles
• A cell-free xylan synthesizing enzyme system from Avena sativa
• Role of lipid in the cellulose synthetase enzyme system from oat seedlings

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