INDUCTION OF GLUTATHIONE S-TRANSFERASE BY PHENOBARBITAL IN RAT HEPATOCYTE CULTURE
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概要
- 論文の詳細を見る
The in vitro effect of phenobarbital on glutathione S-transferase (GST) activity toward 1-chloro-2,4-dinitrobenzene (CDNB) in rat hepatocyte culture was investigated. Treatment of hepatocytes with a medium containing 6 or 8 mM phenobarbital for 20 h increased the specific activity of GST approx. 140% and 160% of control, respectively. When induction of GST by phenobarbital treatment was observed, the cellular level of phenobarbital determined by HPLC was approx. 10-15 μg/mg of 20000×g supernatant protein of hepatocytes. Thus, an inductive effect of phenobarbital on GST was observed in vitro as well as in rat liver. It was suggested that a certain level of cellular phenobarbital was necessary for the induction.
- 公益社団法人日本薬学会の論文
著者
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ISONO Hideo
Faculty of Pharmaceutical Sciences, Teikyo University
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SASAKI HARUYO
Faculty of Pharmaceutical Sciences, Teikyo University
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Isono H
Teikyo Univ. Kanagawa Jpn
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Isono Hideo
Faculty Of Pharmaceutical Sciences Teikyo University
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Sasaki Haruyo
Faculty Of Pharmaceutical Sciences Teikyo University
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MIYAURA SHUICHI
Faculty of Pharmaceutical Sciences, Toikyo University
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Miyaura S
Teikyo Univ. Kanagawa Jpn
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Miyaura Shuichi
Faculty Of Pharmaceutical Sciences Teikyo University
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HORIE Kiyoko
Faculty of Pharmaceutical Sciences, Teikyo University
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Horie Kiyoko
Faculty Of Pharmaceutical Sciences Teikyo University
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