Densitometric Monitoring of Rat Liver Glutathione S-Transferase Activity toward Sumithion and Malathion by the Detection of S-Methylglutathione

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The present work was carried out in order to characterize the glutathione S-transferase involved in the detoxication of Sumithion and Malathion. Rat liver 20000×g supernatant fraction was chromatographed on DEAE- or CM-cellulose column. All the fractions were monitored for enzyme activity toward both pesticides by thin-layer chromatodensitometry after separation of S-methylglutathione on microcolumns of activated carbon (7 mm by 7 mmφ). Glutathione S-transferase B was found to be mainly responsible for the formation of S-methylglutathione from both pesticides, though the activity patterns were a little different. Ligandin, unlike glutathione S-transferase B, was strongly induced in male and female rats by administration of phenobarbital and was also partly responsible for the formation of S-methylglutathione. However, the activity of Ligandin was significantly inhibited by sebacic acid. A DEAE-cellulose column, pH 8.0,was found to retain small amounts of glutathione S-transferases capable of catalyzing the formation of S-methylglutathione from both pesticides.
公益社団法人日本薬学会の論文
1984-12-31