Binding of Paraquat with Water-Insoluble Tissue Preparations by Sephadex G-25 Thin-Layer Chromatography

概要

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The present study was aimed at monitoring tissue materials for their binding ability toward paraquat (PQ : N, N'-dimethyl-γ, γ'-dipiridilium dichloride). Mixtures of PQ with various water-insoluble tissue samples were chromatographed on a Sephadex G-25 thin-layer plate. On the chromatographed plate covered with a sheet of cellophane was spread a solution of sodium hydrosulfite in 1 N sodium hydroxide solution for the detection of PQ as a blue spot. PQ remaining in the original location was considered to be in a state of binding with the tissue materials. Such tissue samples as brain, testis, thymus, pancreas, and submaxillary gland were found to have high ability to bind PQ. On the contrary, Harderian gland, cecum, and seminal vesicle sample, etc. failed to exert any binding ability. This binding mode was suggested to be based on ion exchange reaction, and largely governed by factors of ion strength and pH value of medium, and the fat content in tissue materials.
公益社団法人日本薬学会の論文
1990-02-28