Studies on Protection by Glutathione against Lipid Peroxidation in Rat Liver Microsomes. Effect of Bromosulfophthalein

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The effect of bromosulfophthalein (BSP), an inhibitor of cytosolic glutathione (GSH) S-transferases, on GSH-dependent protection against lipid peroxidation in rat liver microsomes was studied. Microsomal lipid peroxidation induced by ferrous-reduced incotinamide adenine dinucleotide phosphate (NADPH-Fe^<2+>) or ascorbate was prevented by GSH, and addition of BSP abolished the protective effect of GSH in both peroxidation systems. The effect of BSP seemed to occur at concentrations which inhibited the activity of GSH S-transferase in microsomes.The liberation of free fatty acid hydroperoxides from microsomes during lipid peroxidation occurred at pH 7.5-8.0.The rate of NADPH oxidation in the system containing peroxidized microsomes, NADPH, GSH, and GSH reductase was significantly higher than that in the system containing normal microsomes, and was inhibited dramatically by BSP and moderately by phospholipase A_2 inhibitors.The above findings suggest that microsomal GSH S-transferase may be responsible for GSH-dependent protection against peroxidation, probably via radical scavenging and the cooperative action of microsomal phospholipase A_2 and GSH peroxidase activity, which is associated with GSH S-transferase.
社団法人日本薬学会の論文
1986-10-25