Decrease in Sensitivity to Ethidium Bromide by Caffeine, Dimethylsulfoxide or 3-Aminobenzamide Due to Reduced Permeability
スポンサーリンク
概要
- 論文の詳細を見る
The sensitivity of Chinese hamster ovary cells to ethidium bromide, a deoxyribonucleic acid (DNA)-intercalating cationic dye, was reduced in the presence of caffeine, dimethylsulfoxide (DMSO) or 3-aminobenzamide (3AB). As drug resistance is frequently attained by decreased permeability of the drug, the effects of caffeine, DMSO and 3AB on the intracellular accumulation of ethidium were examined. These chemicals were found to decrease the intracellular dose of ethidium via their suppressing effects on the dye uptake with no effect on the efflux. The dye uptake was also suppressed by ouabain and K^+, suggesting that ethidium was taken up by the K^+ transport system. Although the exact mechanism implicated in the inhibitory effects of caffeine, DMSO and 3AB on the dye uptake is not yet clear, these chemicals may interfere with this transport system. The rate of decrease in intracellular dose caused by caffeine, DMSO and 3AB correlated well with that of the reduction in respective cellular sensitivity. This strongly suggests that the decrease in intracellular dye dose in the presence of these chemicals is mainly responsible for the reduced sensitivity. Caffeine, DMSO and 3AB caused a decrease in the binding of ethidium to DNA in vitro. The release of ethidium from DNA molecules may lessen DNA damage, thereby contributing to the reduction in cellular sensitivity to ethidium. However, correlation between inhibitory effects of these chemicals on the dye binding and those on cellular sensitivity is not as good as that between the uptake and the sensitivity. This indicates that effects on dye binding to DNA may not be a major reason for the reduction of cellular sensitivity to ethidium.
- 公益社団法人日本薬学会の論文
著者
-
Kimura Hiroshi
Department Of Anatomy Shiga University Of Medical Science
-
Kimura Hiroshi
Department Of Experimental Radiology Shiga University Of Medical Science
-
Aoyama Takashi
Department Of Computational Science Kanazawa University
-
Kimura Hiroshi
Department Of Anatomy Ii Shiga University Of Medical Science
関連論文
- A New Aspect of the Carotid Body Function Controlling Hypoxic Ventilatory Decline in Humans
- The scid Mutation Does Not Affect Slowly Repairing Potentially Lethal Damage That Is Sensitive To 0.23 M NaCl.
- Scid mutation affects the rate of PLD repair
- Mental Stress Reduces Coronary Flow Velocity Reserve
- Membrane Localization of Protein-Tyrosine Phosphatase 1B is Essential for its Activation of Sterol Regulatory Element-Binding Protein-1 Gene Expression and Consequent Hypertriglyceridaemia
- Properties of Monolithic Silica Columns for HPLC
- THE ORIGIN AND THE EVOLUTION OF THE THIOESTER-CONTAINING PROTEIN(Physiology,Abstracts of papers presented at the 74^ Annual Meeting of the Zoological Society of Japan)
- FUNCTIONAL INVESTIGATION OF THE DRP/DHP/CRAM/UNC-33 FAMILY IN PLANARIANS, DUGESIA JAPONICA(Developmental Biology,Abstracts of papers presented at the 74^ Annual Meeting of the Zoological Society of Japan)
- Possibility of Preventive Treatment for EBV-associated NK Cell-lineage Proliferative Disorders
- A Unified Interpretation of Structures of Liquid Se and Te : Molecular Dynamics Study
- RESULTS OF MULTIPLE CORONARY ARTERY BYPASS GRAFTING IN JAPANESE PATIENTS
- CORONARY ARTERY BYPASS GRAFTING BY UTILIZING THE INTERNAL MAMMARY ARTERY GRAFT IN 100 JAPANESE PATIENTS
- Statistical Associations between Radiation Exposure and the Clinical Examination Data of Japanese Radiology Technicians
- Trial of intraventricular ribavirin therapy for subacute sclerosing panencephalitis in Japan
- Primary Cerebral Angiitis Containing Marked Xanthoma Cells With Massive Intraparenchymal Involvement : Case Report
- Drug-Induced Hypersensitivity Syndrome due to Mexiletine Associated with Human Herpes Virus 6 and Cytomegalovirus Reactivation
- Postnatal development of Serotonergic terminal in rat cerebral cortex
- Ontogenesis of 5HT immunoreactive neurons in the rat brain
- Increase of bone mineral contents in rat tibia after irradiation
- Oral Vitamin C restores impaired Coronary Flow Reserve in Smokers
- Circadian Variation in Coronary Flow Reserve is disappeared in Coronary Heart Disease
- CD4- and CD56-positive T-cell line MTA, established from natural killer-like T-cell leukemia/lymphoma
- 32. On the Experience of the Brachial Vertebral Angiography
- 29. A surgical case of an enormous acoustic tumor with atypical symtoms.
- 72. Experimental studies of hypothermia from the neurosurgical point of view. IV.
- 70. Experimental studies of hypothermia from the neurosurgical point of view. II.
- Neurocysticercosis as Solitary Parenchymal Lesion Confirmed by Mitochondrial Deoxyribonucleic Acid Sequence Analysis : Case Report
- Synthesis of Optically Active Polyarylates Having Axially Chiral Binaphthylene Unit
- Biosynthesis of Poly(3-hydroxyalkanoate)s with Terminal Alkene Groups in the Side Chains by Chromobacterium sp.
- Biosynthesis of Poly(3-hydroxyalkanoate)s with Terminal Alkene Groups in the Side Chains by Chromobacterium sp.
- Production of Polyester Blend of Poly(3-hydroxybutyrate-co-4-hydroxybutyrate) and Poly(3-hydroxyalkanoate) with Saturated and Unsaturated Monomers from 4-Hydroxybutyric Acid by Chromobacterium sp.
- Synthesis and Biodegradation of Polyesters Based on O - Methylene Tartaric Acid
- Effective Biosynthesis of Poly(3-hydroxybutyrate) from Plant Oils by Chromobacterium sp.
- Synthesis and Photoinduced Transformation of a Helical Aromatic Polyamide Having Optically Active Binaphthol Units in the Main Chain
- Biosynthesis of Terpolythioesters with 3-Mercaptopropionate Unit
- Purificaiton and characterization of Secretory-Type GDP-L-Fucose: β-D-Galactoside 2-α-L-Fucosyltransferase from Human Gastric Muscosa
- Hemiconvulsion-hemiplegia syndrome and primary human herpesvirus 7 infection
- Early changes in bone mineral content caused by acute irradiation are not observed after daily fractionation: A study dual-energy X-ray absorptiometry
- Postmortem computed tomography findings as evidence of traffic accident-related fatal injury
- Successful Treatment of Neonatal Herpes Simplex-Type 1 Infection Complicated by Hemophagocytic Lymphohistiocytosis and Acute Liver Failure
- Multiplex real-time PCR for the simultaneous detection of herpes simplex virus, human herpesvirus 6, and human herpesvirus 7
- Comparison of Real-Time and Nested PCR Assays for Detection of Herpes Simplex Virus DNA
- Investigation of Varicella-Zoster Virus DNA in Lymphocyte Subpopulations by Quantitative PCR Assay
- Prevalence of Maternal Cytomegalovirus (CMV) Antibody and Detection of CMV DNA in Amniotic Fluid
- FIBRONECTIN-LIKE IMMUNOREACTIVE STRUCTURES IN EMBRYONIC RAT BRAIN (Modern Histo- and Cytochemistry in Neuroscience : Embryogenesis and Regeneration of the Nervous System)
- Immunohistochemical Demonstration of Fibronectin in the Rat Embryo.
- Arterial and Mixed Venous Oxygen Desaturation during Incremental Exercise in Patients with Chronic Pulmonary Disease
- Control of Upper Airway Function in Response to Hypoxia in Patients with Obstructive Sleep Apnea Syndrome
- Compensatory Excretion of Prostacyclin and Thromboxane Metabolites in Obstructive Sleep Apnea Syndrome
- Molecular analysis of neocarzinostatin induced mutations at the hprt locus
- Analysis of Heart Rate Profile during Obstructive Sleep Apnea
- Detection of Herpesvirus DNA in the Serum of Immunocompetent Children
- Chronic active Epstein-Barr virus infection with dilatation of the Valsalva sinus
- Proposed categorization of pathological states of EBV-associated T/natural killer-cell lymphoproliferative disorder (LPD) in children and young adults : Overlap with chronic active EBV infection and infantile fulminant EBV T-LPD
- -316- IONIC ALTERATIONS IN MYOCARDIAL CELLS DURING OPEN HEART SURGERY STUDIED BY X-RAY MICROANALYSIS
- Combination therapy with intraventricular interferon-α and ribavirin for subacute sclerosing panencephalitis and monitoring measles virus RNA by quantitative PCR assay
- Hepatic parenchymal transection using ultrasonic coagulating shears : a preliminary report
- In Process Nanocrystalline Control Consolidation of the Amorphous ZrO_2-20mol%Al_2O_3 Powder
- Bleomycin induced potentially lethal damage (PLD) expressible by hypertonicity in Chinese haJnster V79 cells.
- Immunocytochemical demonstration of serotonin (5HT) in the human platelet.
- Taurine-like Immunoreactive Structures in the Mouse Liver
- Immunohistochemical demonstration of 6-HT-containing neurons in the CNS.
- Transient Up-regulation of Myotonic Dystrophy Protein Kinase-binding Protein, MKBP, and HSP27 in the Neonatal Myocardium
- Genetic analysis of the STR system D20S161 in Japanese and Bangladeshi
- Imunohistochemical Demonstration of Fibronectin in Rat Brains Lesioned with 5,6-dihydroxytryptamine (5,6DHT).
- Viral Load in Epstein-Barr Virus-Associated Hemophagocytic Syndrome
- ELECTRON MICROSCOPIC ALTERATIONS IN NORADRENALINE NERVE TERMINALS IN VAS DEFERENS OF THE RAT FOLLOWING CHRONIC ADMINISTRATION OF RESERPINE AND CHLORPROMAZINE
- POSTNATAL ALTERATIONS IN NORADRENALINE NERVE TERMINALS IN THE PERIVENTRICULAR HYPOTHALAMUS OF THE RAT
- Histofluorescence method for vizualization of central monoamine neurons in pre- and neonatal rat by glyoxylic acid.
- Extrapancreatic Proinsulin/Insulin-expressing Cells in Diabetes Mellitus : Is History Repeating Itself?
- Association of Cytomegalovirus with Infantile Hepatitis
- The polymorphisms of fucosyltransferases
- A Sensitive Wet-Histofluorescence Method by Glyoxylic Acid for Central Catecholamine Terminals
- Cell transformation from ameboid cells to microglia in the neonatal rats injected fluorescent dye.
- Electron microscopic observation of noradrenergic nerve terminals in the dorsal horn of rat spinal cord (II)
- Divergent Projection of Catecholamine Neuron of Locus Ceruleus as revealed by Fluorescent Retrograde Double Labeling Technique
- Double Labeling Technique of Central Catecholamine Neuron by means of Fluorescent Dyes
- ALTERATION OF DOPAMINE AND TRH IN RAT BRAIN DURING HYPOTHERMIA
- Concurrent Subarachnoid Hemorrhage Due to Ruptured Aneurysm and Hypertensive Intracerebral Hemorrhage : Case Report
- Comparison of the Sauve-Kapandji procedure and the Darrach procedure for the treatment of rheumatoid wrists
- Unrelated Bone Marrow Transplantation Induced Long-Term Remission in a Patient with Life-Threatening Epstein-Barr Virus-Associated Hemophagocytic Lymphohistiocytosis
- Distinctive distribution of AIM1 polymorphism among major human populations with different skin color
- IMMUNOHISTOCHEMICAL AND BIOCHEMICAL STUDIES OF SUBSTANCES WITH TAURINE-LIKE IMMUNOREACTIVITY IN THE BRAIN
- A New Technique for Immunohistochemistry Using Hybrid IgG. : I. Procedure of Hybrid IgG Production.
- Production of new type antisera to thyrotropin releasing hormone (TRH) using carbodiimide coupling and the application to immunohistochemistry.
- SILVER IMPREGNATION AND FLUORESCENCE HISTOCHEMISTRY OF THE SUBEPENDYMAL CELLS IN THE PREOPTIC RECESS OF THE BULLFROG : Rana Catesbeiana
- A fatal case of hyperthermia due to tricyclic antidepressant intoxication
- Myocardial "elektive disseminierte Parenchymnekrose" revisited
- Immunohistochemistry of Serotonin (5-HT) Neurons in the Central Nervus System
- Orthograde transport of horseradish peroxidase (HRP) in central nervous system of young rat.
- Helicobacter pylori infection in patients with idiopathic short stature
- IMMUNOHISTOCHEMICAL STUDY OF FIBRONECTIN IMMUNOREACTIVE STRUCTURES DEMONSTRATED IN RESPONSE TO BRAIN INJURY OF ADULT RATS
- Dynamics and Control of Intelligent Jig with Function of Manipulation
- ALTERATIONS IN NORADRENALINE NERVE TERMINALS IN THE PERIVENTRICULAR HYPOTHALAMUS OF THE RAT DURING REGENERATION AFTER ADMINISTRATION OF 6-OH DOPAMINE
- Mechanical Characteristics of Nanocrystalline (ZrO_2・20mol%Y_2O_3)_(Al_2O_3)_ Synthesized via Pulse Electric Current Consolidation of the Amorphous Powder
- Multicenter evaluation of prototype real-time PCR assays for Epstein-Barr virus and cytomegalovirus DNA in whole blood samples from transplant recipients
- Decrease in Sensitivity to Ethidium Bromide by Caffeine, Dimethylsulfoxide or 3-Aminobenzamide Due to Reduced Permeability
- Biosynthesis of Polyhydroxyalkanoates from 1, 3-Propanediol by Chromobacterium sp
- Studies on Hadamard matrices with “2-transitive” automorphism groups
- A NEW MICROASSAY METHOD FOR L-GLUTAMIC ACID DECARBOXYLASE (GAD) ACTIVITY