胃酸分泌抑制物質(ガストロン物質)に関する研究その2 : ヒトのダ液からシアロガストロンの抽出精製

概要

論文の詳細を見る
Human saliva was separated into dialyzable and nondialyzable fractiona ; the non-dialyzable fraction was found to possess a powerful activity of inhibiting gastric acid secretion and ulceration. This active substance, designated as sialogastrone, was purified by sequential steps of zinc complex precipitation, acetone fractionation, DEAE-cellulose column chromatography, and gel filtration. The purified sialogastrone so obtained possessed inhibitory activity 300 times that of the original lyophilized saliva. This preparation was found to be almost homogeneous by polyacrylamide-gel electrophoresis and showed electrophoretic mobility different from that of other neutral substances in saliva. This purified sialogastrone contained 58.6% of reducing sugars and 31.5% of protein, but no phosphorus or lipids. Its molecular weight was assumed to be over 150,000 from the result of gel filtration.
社団法人日本薬学会の論文
1969-02-25