日本産かぶとがに(Tachypleus tridentatus)血球プレゲルの抽出とその特異性(第1報)

概要

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The lysate of the amebocytes (the pre-gel), which reacts specifically with endotoxin to form gelation reaction, was extracted from Japanese horseshoe crab (Tachypleus tridetatus). The pre-gels, which were prepared by extracting the amobocytes of Japanese horseshoe crab in aqueous solution with N-ethylmaleinimide as anti-aggregating agent showed the sensitivity of 1/100 of the pre-gels prepared from American horseshoe crab (Limulus polyphmus) in gelation reaction to endotoxin, and the active pre-gels were obtained from 30% of an individual of Japanese horseshoe crab. The specific reactivity of the pre-gels to endotoxin was observed, but the pre-gels did not show reaction with any substance from a living body such as amino acids ; peptides, proteins, saccharides, lipids, nucleic acids and related substances, and amines, and therefore, high specificity was recognized. The gelation reaction showed a parallel correlation with the pyrogenic activity of endotoxin and detoxification of endotoxin with alkaline treatment.
社団法人日本薬学会の論文
1974-03-25