Limulus Lysate(Pre-gel)のゲル化反応に関する研究(第5報)ヒト血漿中のLimulus Lysateゲル化反応阻害物質の精製

概要

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The inhibitory substances of the gelation reaction of limulus lysate were purified from human plasma by a five-step procedure. Two purified fractions, E-1 and E-3,were obtained and the recovery of the inhibitory activities in E-1 and E-3 was 8% and 2%, respectively. When examined with disc gel electrophoresis and immunoelectrophoresis against anti-human plasma antiserum, E-3 was revealed to migrate as a single component corresponding to α_2-globulin, whereas E-1 migrated as a major component corresponding to α_1-globulin and minor components. The major electrophoretic component of E-1 was found to represent 83% of the protein by gel scanning. The major component of E-1 gave a precipitin line against anti-human α_1-antitrypsin antiserum, and E-3 against anti-human antithrombin III antiserum by Ouchterlony immunodiffusion and immunoelectrophoresis. The results of molecular weight, carbohydrate, and amino acid analyses of E-1 and E-3 were similar to those of α_1-antitrypsin and antithrombin III, respectively. Purified α_1-antitrypsin and antithrombin III were also recognized to inhibit the gelation reaction of limulus lysate. These results soggest that the inhibitory substances of the gelation reaction of limulus lysate in human plasma are α_1-antitrypsin and antithrombin III and that most of the inhibitory activity is attributable to α_1-antitrypsin.
社団法人日本薬学会の論文
1980-05-25