微生物定量法による尿中の遊離アミノ酸定量における尿素の影響

スポンサーリンク

概要

• 論文の詳細を見る

• Previous result on the determination of phenylanine in the urine of phenylketonuria by a microbiological method indicated marked inhibition by the presence of urea and, therefore, effect of urea on the calibration curve of phenylalanine was examined. It was thereby found that even as little as 1mg./ml. of urea affected the value of phenylalanine and that the growth of microorganisms was completely stopped by the presence of 15mg./ml.of urea. It was also found that aseptic filtration would be available as a method of disinfection as this hardly inhibited growth of the microorganisms. Since the use of aseptic filtration for microbiological assay is very inconvenient, an alternative method was devised by treating the samples containing urea with Amberlite CG-120 by which urea was found to be completely removed. A good result was obtained with recovery rate of phenylalanine coming to 100±4%. The recovery rate of phenylalanine is only 81% when urea was removed by acid decomposition. In the case of a sample containing conjugate-type amino acid, this amino acid is liberated by acid decomposition and this method cannot be used when free amino acid alone is to be determined. Therefore, the above resin treatment seems to be a convenient method in such a case.

• 公益社団法人日本薬学会の論文
• 1966-08-25

著者

• 朝川 雅子

  大阪薬科大学

• 川野 タツ

  大阪薬科大学

• 川野 タツ

  Laboratory Of Biochemistry Osaka College Of Pharmacy

関連論文

• Purification and Properties of "N^α-Benzyloxycarbonyl Amino Acid Urethane Hydrolase III"from Lactobacillus casei ε ATCC 7469
• A NOVEL ENZYME "N^α-BENZYLOXYCARBONYL AMINO ACID URETHANE HYDROLASE II" FROM LACTOBACILLUS FERMENTI 36 ATCC 9338
• 微生物定量法による尿中の遊離アミノ酸定量における尿素の影響
• 蚕の最終糞と尿酸の水解物のグリシンについて
• Ultra-microdetermination of Amino Acids by Microbioassay, Applying Lactic Acid Assay with Lactate Dehydrogenase
• 蚕糞水解物のグリシンについて
• 家蚕の糞の遊離アミノ酸について
• 微生物定量法による魚粕中のアミノ酸の定量

スポンサーリンク