高速液体クロマトグラフィによる医薬品の分析(第1報)軟膏中のデキサメタゾン・マレイン酸クロルフェニラミンの同時定量

概要

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Simultaneous determination of chlorpheniramine maleate (MCP) and dexamethasone (DM) in ointment was established by using high performance liquid chromatography. On a μ-Bondapak C_<18> column (4 mm i.d.×300 mm) with an eluent of CH_3OH : H_2O=80 : 20,sufficient separation of MCP, DM and other components in ointment was obtained. MCP and DM in ointment were extracted with CH_3OH. A 2μl portion of the extracted solution with naphthalene as internal standard was injected into the column, and the effluent was monitored by ultraviolet detector (λ=254 nm). Coefficients of variation for the determination of MCP and DM in ointment were found to be less than 0.35%. In comparison to other methods (determination of DM with isoniazid dihydrochloride, spectrophotometric determination of MCP by solvent extraction with zincon), the present method was superior in simplicity and accuracy.
公益社団法人日本薬学会の論文
1979-05-25