生薬主成分の分析化学的研究(第2報)オウレン中ベルベリンの高速液体クロマトグラフィによる定量

概要

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A high performance liquid chromatographic method was developed for the determination of berberine in Coptidis rhizoma and Oriental pharmaceutical preparations. Retention time of berberine (flow rate : 1.0 ml/min) was approximately 4 min 40 sec on a μ-Bondapak C_<18> column (4×300 mm) with an eluent of acetonitrile : phosphate buffer (pH 5.2)=60 : 40. Naphthalene was used as an internal standard, whose retention time was approximately 9 min 36 sec. Berberine was extracted from the samples with methanol and injected into the column. Coefficient of variation for berberine content in Coptidis rhizoma was found to be less than 0.45%. It was concluded from the comparison of this method with three other methods (thin-layer chromatography, thin-layer chromatography densitometry, and gas chromatography) that this method was significantly better than other methods with respect to simplicity, analytical time, and accuracy.
公益社団法人日本薬学会の論文
1977-12-25