家兎の体液と尿中のフロセミドおよび代謝物の高速液体クロマトグラフ法による分析

概要

論文の詳細を見る
Furosemide (FR) was administered to normal and acute renal failure rabbits by an intravenous administration on a single dosing (10 mg/kg). A peak on chromatogram proposed as FR-glucuronide (FG) was detected by high performance liquid chromatography with fluorometric detector. Assuming that difference in FR concentration between samples before and after hydrolysis with β-glucuronidase was attributed to FG, the concentrations of FR and FG were determined, separately. The plasma concentrations of FR and FG in acute renal failure rabbit were higher than those in normal rabbit during a 2-h period after administration of FR. Approximately 11% and 20% of total FR dosing were excreted as FG in 24-h urine of normal and acute renal failure rabbits, respectively. A larger biliary excretion of FG in acute renal failure than in normal could be also observed.
社団法人日本薬学会の論文
1982-12-25